日用化学工业（中英文）2024，Vol.54Issue(1)：107-113,7.DOI:10.3969/j.issn.2097-2806.2024.01.015
UHPLC-MS/MS法测定面膜化妆品中甘草、人参和黄芩类功效成分
Determination of functional components of Glycyrrhizae,Ginseng and Scutellariae in facial masks by UHPLC-MS/MS
摘要
Abstract
An analytical method was established for the determination of Glycyrrhizae functional components(liquiritin and glycyrrhizic acid),Ginseng functional components(ginsenoside Rg1,ginsenoside Rb1,ginsenoside Re)and Scutellariae functional components(baicalin)in facial mask cosmetics with ultra-high performance liquid chromatography triple quadrupole mass spectrometry(UHPLC-MS/MS).This method was used to monitor the risk of 20 batches of samples sold on the market.The samples were extracted by ultrasonic method using 0.1%formic acid in methanol.The mobile phase was 0.1%formic acid aqueous solution-acetonitrile solution,and the gradient elution was carried out.The samples were separated on the ACQUITY UPLC HSS T3(2.1 mm×100 mm,1.8 μm)column and detected by UHPLC-MS/MS negative ion mode.At the same time,the pretreatment conditions and UHPLC-MS/MS conditions were optimized and the method was verified.The results show that the method shows good linearity in the mass concentration range of 0.25-100 ng/mL for liquiritin and baicalin,and 0.50-200 ng/mL for ginsenoside Rg1,ginsenoside Rb1,ginsenoside Re and glycyrrhizic acid,with R no less than 0.998.The limits of detection are in the range of 0.41-6.4 μg/kg(S/N=3).The average recoveries in the sample matrix of facial masks are from 95.9%to 109.1%,with the relative standard deviations(RSDs)less than 4.9%(n=6).This method is suitable for the determination of ginseng functional components in the ginseng cosmetics.关键词
面膜化妆品/甘草/人参/黄芩/功效成分/液质联用Key words
facial masks cosmetics/Glycyrrhizae/Ginseng/Scutellariae/functional components/UHPLC-MS/MS分类
化学化工引用本文复制引用
陆林玲,鲁辉,闵春艳,钱叶飞..UHPLC-MS/MS法测定面膜化妆品中甘草、人参和黄芩类功效成分[J].日用化学工业（中英文）,2024,54(1):107-113,7.基金项目
中国药品监管科学行动计划第二批重点项目"中药有效性安全性评价及全过程质量控制研究"子课题(NMPAJGKX-2023-043) （NMPAJGKX-2023-043）
江苏省药品监督管理局科研计划项目资助(202366) （202366）
