日用化学工业（中英文）2024，Vol.54Issue(1)：114-122,9.DOI:10.3969/j.issn.2097-2806.2024.01.016
高效液相色谱法同时测定化妆品中24种香豆素类化合物及质谱确证
Simultaneous determination of 24 coumarin compounds in cosmetics by high performance liquid chromatography and verification by liquid chromatography-tandem mass spectrometry
摘要
Abstract
An analytical method for simultaneous determination of 24 coumarin compounds in cosmetics was established by high performance liquid chromatography.Samples with certain fluidity,such as lotions,creams,and water-based samples,were directly extracted with methanol by ultrasonic treatment,while solid samples(lipsticks,pressed powders,etc.)were ultrasonically extracted with mixed solvent of tetrahydrofuran-methanol(1∶1).The compounds were separated on a CAPCELL CORE MGII C18(4.6×250 mm,5 μm)column,and gradient elution was carried out with methanol and 0.02 mol/L sodium dihydrogen phosphate buffer(pH 3.5)as the mobile phase.The flow rate was 1.0 mL/min.The quantification was done by external standard method,with PDA detector at multi-wavelength,and the suspected positive samples were confirmed by liquid chromatography-tandem mass spectrometry.The results showed that the 24 coumarin compounds showed good linearity,with correlation coefficient≥0.999 6 in their respective mass concentration range.The limits of detection and limits of quantification were 2-19 mg/kg and 5-63 mg/kg,respectively.At the three spiked levels of low,medium and high,the average recoveries ranged from 94.4%to 106.4%,and the relative standard deviations were between 0.5%and 3.3%.This method was simple,accurate and suitable for the determination of 24 coumarin compounds in cosmetics.关键词
香豆素/化妆品/高效液相色谱法/液相色谱-串联质谱法Key words
coumarin/cosmetic/high performance liquid chromatography/liquid chromatography-tandem mass spectrometry分类
化学工程引用本文复制引用
龙慧端,鲁毅翔,覃江兰,张科明..高效液相色谱法同时测定化妆品中24种香豆素类化合物及质谱确证[J].日用化学工业（中英文）,2024,54(1):114-122,9.基金项目
广西药品监督管理局科研计划(桂药监科2022-(039)) （桂药监科2022-(039）
