四川中医2023，Vol.41Issue(11)：75-80,6.
基于突触可塑性理论研究电针对抑郁模型大鼠行为学及海马突触相关蛋白mTOR、GSK-3β的影响
Study on the Effects of Electroacupuncture on Behavior and Hippocampal Synapse Related Proteins mTOR and GSK-3β in Depression Model Rats Based on Synaptic Plasticity Theory
摘要
Abstract
Objective:Explore the mechanism of acupuncture on treating the depression by observing the effects of electroa-cupuncture(EA)at"Baihui"(GV20)and"Yintang"(GV29)on depressive behavior and hippocampal synapse-related pro-tein mTOR and GSK-3β(Glycogen synthase kinase-3β)of WKY rats(Wistar-Kyoto rats,an animal model of depression)Methods:15 Wistar rats served as normal group.45 WKY rats were randomly divided into EA group,Sham Acupuncture(SA)group and model group.Sucrose Water Preference Test(SPT),Open Field Test(OFT)and body mass were measured in all rats before and after the treatment.EA group were treated with GV20 and GV29,SA group were treated with sham acupuncture.Fed model group and normal group were regularly raised without any intervention.After the treatment,we detect the Protein expression of mTOR and GSK3-βin rat hippocampus synapsis by Western blot.Results:Before the treatment,WKY rats have higher weight and perform better in SPT and OFT,compared with Wistar rats(P＜0.01).After the treatment,EA group have higher weight,SPT rate and OFT rate compare among SA group and model group.(P＜0.01).The expression level of mTOR in hippocampus in EA group was higher,compared among SA group and model group(P＜0.05).The expression level of mTOR in hippocampus in normal group was higher,compared with model group(P＜0.01).The expression level of GSK3-β in hippocampus in EA group was lower,compared among SA group and model group(P＜0.05).The expression level of GSK3-β in hippocampus in normal group was lower,compared with model group(P＜0.01)Conclusion:The depression symptoms of WKY rats were relieved by electroacupuncture treatment.Its mechanism could be related to the up-regulating expression of mTOR in hippocampus and the down-regulating expression of GSK3-β,these changes of protein expression can improve the synaptic plasticity in hippocampus and therefore alleviate depression.关键词
电针/抑郁症/突触可塑性Key words
electroacupuncture/depression/synaptic plasticity分类
医药卫生引用本文复制引用
黄树航,邢龙飞,杨帅,李晓慧,冯荣荣,王子未,秦丽娜..基于突触可塑性理论研究电针对抑郁模型大鼠行为学及海马突触相关蛋白mTOR、GSK-3β的影响[J].四川中医,2023,41(11):75-80,6.基金项目
北京中医药大学第三附属医院科研孵化项目(编号:2019kyth-05) （编号:2019kyth-05）
北京中医药大学校级课题(编号:2020-JYB-XJSJJ-066). （编号:2020-JYB-XJSJJ-066）
