山东农业大学学报（自然科学版）2023，Vol.54Issue(6)：799-807,9.DOI:10.3969/j.issn.1000-2324.2023.06.001
苹果周期蛋白依赖性蛋白激酶基因MdCDKB1的克隆及表达
Cloning and Expressing of Cyclin-dependent Protein Kinase Gene MdCDKB1 in Apple
摘要
Abstract
Cyclin-dependent kinase B(CDKB)is one of cell cycle genes,and controls fruit cell number and fruit size in apple(Malus × domestic Borkh.).In order to explore MdCDKB expression during the development of apple fruit,we used the cultivar'Ralls'and'Fuji'with the difference fruit size as materials,MdCDKB was cloned from pericarp and its expressing was studied during fruit growth stages.The segments were divided into two kinds of sequence,their open reading frames are 915 bp coding 304 amino acids.The maximum consistent sequences of nucleotide and protein were CDKB gene in plants,and the maximum consistent EST sequences are two EST sequences in'Gala'apple:CV085424(CDKB1;1)and EB138473(CDKB1;2).Bioinformatics predictions indicate that its protein sequence includes the motif PPTALRE of the plant cell cycle-dependent protein kinase B1(CDKB 1),belonging to plant CDKB subfamily.Therefore,the two sequences were named respectively MdCDKB1;1 and MdCDKB1;2.The expression peak of MdCDB1;1 and MdCDB1;2 occurred respectively at 2 1d and 7d after full bloom which coincided with rapid growth rate of early fruit,and would play roles during cell division;meanwhile the expressing level and the sustaining period of MdCDB1;1 is much higher and longer than MdCDB1;2.In'Fuji'with larger fruit,the expression level of MdCDB1;1 and MdCDB1;2 are lower than in'Ralls'with small fruit,the expressing level of MdCDB1;1 and MdCDB1;2 have no obvious effect on fruit size difference between'Ralls'and'Fuji'.关键词
苹果/果实/细胞周期/MdCDKB1Key words
Apple/fruit/cell cycle/MdCDKB1分类
农业科技引用本文复制引用
崔美,焦其庆,于鹏,姜远茂,张宗营,陈学森,陈晓流..苹果周期蛋白依赖性蛋白激酶基因MdCDKB1的克隆及表达[J].山东农业大学学报（自然科学版）,2023,54(6):799-807,9.基金项目
农业部公益行业(农业)科研专项基金(201203044) （农业）
