山东农业大学学报（自然科学版）2023，Vol.54Issue(6)：858-864,7.DOI:10.3969/j.issn.1000-2324.2023.06.009
短芽孢杆菌PB1对聚乙烯的降解
Degradation of Polyethylene by Brevibacillus PB1
摘要
Abstract
In order to explore an environmental method for mitigating"white pollution",a polyethylene-degrading bacteria strain PB1 was isolated by enriching and screening the inorganic salt medium with polyethylene as the sole carbon source from the soil covered with polyethylene film.The colony of PB1 was round with neat edge,moist and smoot surface.Additionally,the bacterium is gram strain positive and rod-shaped.The 16S rDNA gene sequence analysis showed that it had more than 99％identity with multiple Brevibacillus strains.Based on these characteristics,the strain PB1 was preliminarily identified as Brevibacillus sp.After co-cultivation of PB1 and polyethylene for 35 days,the weight loss rate of polyethylene membrane was 3.05％,and the bacterial fluid concentration(OD600)in the culture medium showed an increasing trend.Obvious pits and dents on the surface of degraded polyethylene diaphragm were observed under scanning electron microscopy and with filamentous cracks.According to the Fourier infrared transform spectrometer,the enzyme activity of laccase and manganese peroxidase were 0.125 iu·ml～(-1)and 0.126 iu·ml～(-1)at 35 d.It was proved that strain PB1 could degrade polyethene efficiently.Our study will enrich the strain resources of polyethylene-degrading bacteria and lay a foundation for the further study of polyethylene biodegradation.关键词
短芽孢杆菌/聚乙烯/降解Key words
Brevibacillus sp./Polyethylene/degradation分类
农业科技引用本文复制引用
肖梦杰,王怡霏,王桂端,赵欣,罗海龙,朱利霞..短芽孢杆菌PB1对聚乙烯的降解[J].山东农业大学学报（自然科学版）,2023,54(6):858-864,7.基金项目
河南省高等学校重点科研项目(23B180004) （23B180004）
国家自然科学基金(32201414) （32201414）
