山东医药2023，Vol.63Issue(36)：5-9,5.DOI:10.3969/j.issn.1002-266X.2023.36.002
人OC细胞系细胞miR-211-5p表达、转染miR-211-5p模拟物后的增殖及迁移侵袭和EMT观察
Expression of miR-211-5p in human OC cell lines and its regulatory effects on cell proliferation,migration,invasion,and EMT
摘要
Abstract
Objective To observe the expression of microRNA-211-5p(miR-211-5p)in human ovarian cancer(OC)cell lines and the cell proliferation,migration,invasion,and epithelial mesenchymal transition(EMT)in cells after transfection of miR-211-5p mimics.Methods Human OC cell lines(SKOV3,A2780,HO8910 cells)and human nor-mal ovarian epithelial IOSE80 cells were cultured in vitro,and the miR-211-5p in these cells was detected using qRT-PCR.SKOV3 cells in the logarithmic growth phase were selected and randomly divided into two groups.Cells in the high expression group were transfected with miR-211-5p mimics,while cells in the control group were transfected with miR-NC.The OD value,percentage of scratch movement distance,the number of invading cells,gray value ratio of EMT-relat-ed protein and chitosanase 3-like protein 1(CHI3L1)protein were measured by CCK-8 experiment,cell scratch experi-ment,Transwell experiment,and Western blotting,respectively.The miR-211-5p mimics or miR-NC and CHI3L1 wild-type dual luciferase reporter vector CHI3L1-wt or CHI3L1 mutant dual luciferase reporter vector CHI3L1-mut were co-transfected into SKOV3 cells,which were taken as the miR-211-5p mimics+CHI3L1-wt group,miR-NC+CHI3L1-wt group,miR-211-5p mimics+CHI3L1 mut group,and miR-NC+CHI3L1 mut group,respectively.After 24 h of transfec-tion,cells were collected and lysed,and the luciferase activity of cells in each group was detected using the luciferase as-say kit.Results Compared with the IOSE80 cells,the relative expression of miR-211-5p in SKOV3,A2780,and HO8910 cells decreased(all P＜0.05).Compared with the control group,the OD value,the percentage of scratch move-ment distance,the number of invading cells,and gray value ratio of N-Cadherin and Fibronectin protein in the high expres-sion group decreased,while the gray value ratio of E-Cadherin protein increased(all P＜0.05).Compared with the control group,the gray value ratio of CHI3L1 protein in the high expression group decreased(P＜0.05).Compared with the miR-NC+CHI3L1-wt group,the relative luciferase activity decreased in the miR-211-5p mimics+CHI3L1-wt group(P＜0.05).Conclusion The expression of miR-211-5p is reduced in OC cell lines;transfection with high expression miR-211-5p can inhibit SKOV3 cell proliferation,migration,invasion,and EMT,and the mechanism is achieved through negative reg-ulation of CHI3L1 expression.关键词
微小核糖核酸-211-5p/卵巢癌/SKOV3细胞/细胞增殖/细胞迁移/细胞侵袭/细胞上皮间质转化/壳多糖酶3样蛋白1Key words
microRNA-211-5p/ovarian carcinoma/SKOV3 cells/cell proliferation/cell migration/cell inva-sion/epithelial-mesenchymal transition of cells/chitosanase 3-like protein 1分类
医药卫生引用本文复制引用
杨立芬,何建清,尹立新..人OC细胞系细胞miR-211-5p表达、转染miR-211-5p模拟物后的增殖及迁移侵袭和EMT观察[J].山东医药,2023,63(36):5-9,5.基金项目
河北省医学科学研究课题计划(20191534). （20191534）
