甲基转移酶样蛋白14对急性早幼粒白血病细胞增殖、凋亡、侵袭和迁移的调节作用OACSTPCD

Objective To investigate the effects of overexpression and knockdown of methyltransferase-like protein 14(METTL14)on proliferation,apoptosis,invasion and migration of acute promyelocytic leukemia cells.Methods Human acute promyelocytic leukemia cells NB4 were cultured,and were divided into the overexpression control(Over-NC)group,overexpression group,interference control(si-NC)group,and interference group,respectively.The blank plasmid and METTL14 plasmid were transfected into NB4 cells in the control group and the overexpression group,respec-tively.The si-NC and si-METTL14 were transfected into NB4 cells in the interference control group and the interference group,respectively.Cell proliferation was detected by CCK-8,apoptosis was detected by flow cytometry,cell invasion and migration abilities were detected by Transwell chamber,and N-6 methyladenine(m6A)modification level was detect-ed by Dot blotting assay.Results Compared with the Over-NC group,the cell proliferation rate increased,apoptosis rate decreased,cell migration number and cell invasion number increased,and m6A modification level of NB4 cells in-creased in the overexpression group(all P<0.05).Compared with the si-NC group,the proliferation rate decreased,apop-tosis rate increased,cell migration number and cell invasion number decreased,and the m6A modification level of NB4 cells decreased in the interference group(all P<0.05).Conclusion METTL14 can promote the proliferation,migration and invasion of NB4 cells,and its mechanism may be related to the increase of m6A modification level.