山东医药2024，Vol.64Issue(2)：31-34,38,5.DOI:10.3969/j.issn.1002-266X.2024.02.007
CDC25C表达下调的黑色素瘤细胞线粒体应激反应和线粒体途径凋亡情况观察
Effects of down-regulation of CDC25C on mitochondrial stress response and mitochondrial pathway apoptosis in melanoma cells
摘要
Abstract
Objective To investigate the effects of cell division cycle 25C(CDC25C)down-regulation on the mito-chondrial stress response and mitochondrial pathway apoptosis in melanoma B16 cells.Methods B16 cells were cul-tured and randomly divided into the transfection group,transfection control group and blank control group.In the transfec-tion group,B16 cells were transfected with the lentiviral plasmid with low-expression of CDC25C.In the transfection con-trol group,B16 cells were transfected with the lentiviral empty plasmid,while in the blank control group,B16 cells were cultured normally.Rhod-2/AM and MitoSOX fluorescence probe were used to detect the concentration of mitochondrial Ca2+ and mitochondrial reactive oxygen species(ROS)in B16 cells.The mRNA and protein expression levels of mitochon-drial stress response-related molecules mitochondrial caseinolytic protease P(ClpP)and Lon protease 1(LONP1),as well as the mitochondrial pathway apoptosis-related molecules Caspase-9 and Caspase-3 were detected using RT-qPCR and Western blotting.Results Compared with the transfection control group and the blank control group,the concentration of mitochondrial Ca2+ and mitochondrial ROS in B16 cells increased,and the mRNA and protein expression levels of ClpP,LONP1,and Caspase-9,and Caspase-3 increased in the transfection group(P<0.05 or P<0.01).Conclusion Down-regulation of CDC25C in melanoma B16 cells activates the mitochondrial stress response and subsequently triggers mito-chondrial pathway apoptosis.关键词
黑色素瘤/细胞分裂周期蛋白25同源蛋白C/线粒体应激反应/线粒体钙离子超载/线粒体活性氧/细胞凋亡Key words
melanoma/cell division cycle 25C/mitochondrial stress response/mitochondrial calcium overload/mitochondria reactive oxygen species/apoptosis分类
医药卫生引用本文复制引用
缪欣宇,栗彦飞,郑方园,刘海龙,张瑶尧,晁耐霞,莫发荣..CDC25C表达下调的黑色素瘤细胞线粒体应激反应和线粒体途径凋亡情况观察[J].山东医药,2024,64(2):31-34,38,5.基金项目
广西自然科学基金项目(2023GXNSFAA026070,2018GXNSFAA281071). （2023GXNSFAA026070,2018GXNSFAA281071）
