上海针灸杂志2024，Vol.43Issue(1)：81-88,8.DOI:10.13460/j.issn.1005-0957.2023.13.3006
降钙素基因相关肽在胃溃疡模型小鼠穴位敏化中的作用研究
Study of the Role of calcitonin gene-related peptide in acupoint sensitization in gastric ulcer mice
摘要
Abstract
Objective To discuss the role of calcitonin gene-related peptide(CGRP)in acupoint sensitization in chronic gastric ulcer mice from the perspective of molecular biology.Method C57BL/6 mice were randomly divided into a control group and a model group,with 15 mice in each group.The chronic gastric ulcer model was established by injection of glacial acetic acid into the submucosal layer of the gastric wall near the pylorus in the lesser curvature,followed by Evans blue injection into the tail vein of mice to observe the distribution pattern of acupoint sensitization spots on the body surface.Pathological changes in gastric tissues after modeling were observed by hematoxylin-eosin staining.Changes in the pain threshold of mice were observed by body surface hyperalgesia-like behavior tests.The expression of CGRP in the spinal cord,dorsal root ganglion(DRG),and skin after acupoint sensitization was detected by Western blotting and immunofluorescence staining.Result Injecting glacial acetic acid into the stomach of mice could induce a model conforming to the typical pathological features of gastric ulcer.The control group had fewer EB exudate spots,while the model group had EB exudate spots scattered in the dominant regions of T1-T13 segments of the spinal cord,predominantly concentrated in the T9-T11 regions,and a significantly lower pressing pain threshold was found at the Evans blue exudate spots compared with that before modeling(P＜0.01).After acupoint sensitization,the CGRP protein levels and positive expression of fluorescent protein in the spinal cord,DRG,and skin were significantly higher in the model group than in the control group(P＜0.01).Conclusion After the establishment of gastric ulcers induced by glacial acetic acid in mice,the expression levels of CGRP in the sensitized regions on the body surface,the corresponding T9-T11 spinal cord segments,and DRG neurons corresponding to the gastric lesions were significantly accelerated,which may be responsible for peripheral and central hyperalgesia,topical neurogenic inflammatory responses on the body surface,and mediating acupoint sensitization.关键词
胃溃疡/穴位敏化/痛阈/降钙素基因相关肽/小鼠Key words
Stomach ulcer/Acupoint sensitization/Pain threshold/Calcitonin gene-related peptide/mice分类
医药卫生引用本文复制引用
杨雪,郑璇,袁伟,吴宇蔚,刁志君,薛思梦,乔海法..降钙素基因相关肽在胃溃疡模型小鼠穴位敏化中的作用研究[J].上海针灸杂志,2024,43(1):81-88,8.基金项目
国家自然科学基金项目(81873385,82074557) （81873385,82074557）
陕西省教育厅项目(18JK0213) （18JK0213）
陕西省科技厅项目(2019JM-399) （2019JM-399）
