上海针灸杂志2024，Vol.43Issue(1)：89-95,7.DOI:10.13460/j.issn.1005-0957.2024.01.0089
经颅重复针刺对血管性痴呆模型大鼠认知功能及海马突触超微结构的影响
Effects of repetitive transcranial acupuncture on cognitive function and hippocampal synaptic ultrastructure in rats with vascular dementia
摘要
Abstract
Objective To observe the effects of repetitive transcranial acupuncture on the cognitive function and the expression of synaptophysin(SYN)and microtubule associated protein-2(MAP-2)in hippocampal CA1 region of vascular dementia(VaD)rats.Method Fifty-six SD rats were randomly divided into a blank control group(14 rats)and a model group(42 rats).VaD model was established in the model group,and the successful model rats were randomly divided into a model group(14 rats),a conventional acupuncture group(14 rats)and a repetitive transcranial acupuncture stimulation(rTAS)group(14 rats).On the second day after successful modeling,the rTAS group was treated with repetitive transcranial acupuncture.The rats in the conventional acupuncture group only received conventional acupuncture manipulation.The rats in the model group and the blank control group were grasped and fixed under the same conditions without acupuncture.Water maze was used to evaluate the learning and memory ability of rats,and open field test was used to evaluate the exploratory behavior and autonomous activity ability of rats.Western blot was used to detect the expression of SYP and MAP-2 protein in the hippocampal CA1 region,and transmission electron microscope was used to observe the synaptic ultrastructure of hippocampus.Result Compared with the blank control group,the escape latency time of the model group was significantly increased(P＜0.05),and the frequency of crossing the platform was significantly reduced(P＜0.05).Compared with the model group,the conventional acupuncture group and the rTAS group had a significant reduction in the escape latency time(P＜0.05)and a significant increase in the number of platform crossings(P＜0.05).Compared with the routine acupuncture group,the rTAS group had no significant changes in the escape latency time and the number of platform crossings(P＞0.05).Compared with the blank control group,the number of crossing grids and the number of hind limb standing in the model group were significantly reduced(P＜0.05).Compared with the model group,the conventional acupuncture group and the rTAS group had significant increases in the number of grids crossing and the number of hind limbs standing(P＜0.05).Compared with the conventional acupuncture group,the rTAS group had significant increases in the number of crossing grids and the number of hind limb standing(P＜0.05).Compared with the blank control group,the expression levels of SYP and MAP-2 in the model group were significantly decreased(P＜0.05).Compared with the model group,the expression levels of SYP and MAP-2 in the conventional acupuncture group and the rTAS group were significantly increased(P＜0.05).Compared with the conventional acupuncture group,the rTAS group had significant increases in the expression levels of SYP and MAP-2(P＜0.05).Under electron microscope,the synaptic structure of the model group was not clear,the synaptic gap was blurred,the synaptic vesicles were fewer,the presynaptic dense material was reduced,the organelles in the cytoplasm were sparse,and the mitochondria were deformed and enlarged obviously.In rTAS group,the synaptic structure was more complete,the synaptic cleft,the boundary between the pre-and post-synaptic membranes were clear,there were more vesicles in the presynaptic membrane,the postsynaptic membrane was evenly thickened,and the surrounding mitochondria were abundant and structurally intact.Conclusion Compared with conventional acupuncture,repetitive transcranial acupuncture may promote synaptic regeneration,improve synaptic plasticity,repair damaged neurons,and improve cognitive function by regulating the expression of SYP and MAP-2.关键词
针刺疗法/经颅重复针刺/痴呆,血管性/突触/海马/认知功能/大鼠Key words
Acupuncture therapy/Repeated transcranial acupuncture/Dementia,vascular/Synapses/Hippocampus/Cognitive function/Rats分类
医药卫生引用本文复制引用
于国强,张良,李红伟,孙婧妍,关莹,唐祎周,杨添淞,石光煜,冯秋菊,曹燚,吴壮..经颅重复针刺对血管性痴呆模型大鼠认知功能及海马突触超微结构的影响[J].上海针灸杂志,2024,43(1):89-95,7.基金项目
国家自然科学基金面上项目(82074539) （82074539）
黑龙江省卫生健康委科研项目(20211212010322) （20211212010322）
孙申田青年人才基金(2020KY-12) （2020KY-12）
黑龙江省国医大师学术思想传承科研课题项目(GY2022-19) （GY2022-19）
