世界中医药2023，Vol.18Issue(22)：3207-3210,4.DOI:10.3969/j.issn.1673-7202.2023.22.008
龟羚帕安丸对帕金森病大鼠转录因子EB介导自噬调控机制的研究
Effect of Guiling Pa'an Pill on the Regulation Mechanism of Transcription Factor EB-mediated Autophagy in Parkinson's Disease Rats
摘要
Abstract
Objective:To study the effect of Guiling Pa'an Pill on the expression of transcription factor EB(TFEB)in the treat-ment of Parkinson's disease(PD)and its mechanism.Methods:The PD rat model was randomly divided into model group,western medicine group,traditional Chinese and western medicine group and traditional Chinese medicine low,middle and high dose groups,with 15 in each group.Another 15 normal rats were selected as sham operation group.The model group and sham operation group were given same volume of normal saline,and the other groups were given corresponding medicines by intragastric administra-tion for 4 consecutive weeks.After the PD rats were sacrificed,the substantia nigra of the midbrain was collected.The protein ex-pressions of TFEB,microtubule-associated protein light chain 3 Ⅱ(LC3 Ⅱ),P62,tyrosine hydroxylase(TH)and α-synuclein(α-syn)were detected by Western Blotting.Results:Compared with the conditions in sham operation group,the protein expressions of TFEB,LC3 Ⅱ and TH in the substantia nigra of the model group,western medicine group,traditional Chinese and western medicine group and traditional Chinese medicine groups decreased significantly(P＜0.01),while the protein expressions of P62 and α-syn increased significantly(P＜0.01).Compared with the model group,the western medicine group,traditional Chinese and western medicine group and traditional Chinese medicine groups had up-regulated TFEB,LC3 Ⅱ and TH in substantia nigra(P＜0.01),while down-regulated P62 and α-syn(P＜0.01).Conclusion:Guiling Pa'an Pill can activate autophagy-lysosome system through TFEB,enhance the autophagy of neural cells,increase LC3 Ⅱ and TH,and promote the degradation and clearance of α-syn and P62,thereby playing a neuroprotective effect.关键词
帕金森病/龟羚帕安丸/自噬/转录因子EB、微管相关膜蛋白轻链3Ⅱ、泛素结合蛋白P62、酪氨酸羟化酶、α-突触核蛋白Key words
Parkinson's disease/Guiling Pa'an Pill/Autophagy/Transcription factor EB/Microtubule-associated protein light chain 3 Ⅱ/P62/Tyrosine hydroxylase/α-synuclein分类
医药卫生引用本文复制引用
陈帅杰,胡慧慧,常学辉,张良芝,郭健,张创业,王冬莲..龟羚帕安丸对帕金森病大鼠转录因子EB介导自噬调控机制的研究[J].世界中医药,2023,18(22):3207-3210,4.基金项目
全国名老中医药专家传承工作室建设项目(国中医药人教函[2022]75号) （国中医药人教函[2022]75号）
河南省科技攻关项目(222102310630) （222102310630）
全国名老中医药专家传承工作室建设项目[豫卫中医函(2022)3号] （2022）
河南省卫健委-国家中医临床研究基地科研专项(2021JDZX2065) （2021JDZX2065）
河南省中医药科学研究专项课题(2019ZYBJ16) （2019ZYBJ16）
河南中医药大学研究生科研创新类项目(2021KYCX024) （2021KYCX024）
