生命科学研究2023，Vol.27Issue(6)：479-487,9.DOI:10.16605/j.cnki.1007-7847.2022.09.0210
miR-144-3p靶向E2F8抑制肺腺癌细胞的增殖和侵袭并阻滞细胞周期
miR-144-3p Inhibits Proliferation and Invasion of Lung Adenocarcinoma Cells and Arrests Cell Cycle by Targeting E2F8
宋世伟 1李景武 2刘晓辉3
作者信息
- 1. 唐山市人民医院胸外科,中国 河北唐山 063000
- 2. 唐山市人民医院胃肠外科,中国 河北唐山 063000
- 3. 唐山市人民医院胸外二科,中国 河北唐山 063000
- 折叠
摘要
Abstract
To investigate the mechanism of miR-144-3p in lung adenocarcinoma(LUAD),the expression le-vels of miR-144-3p and its downstream target gene in LUAD tissues were predicted based on multiple databases.Through bioinformatics methods and based on the related literature,E2F transcription factor 8(E2F8)was identified as the target gene of miR-144-3p,and the dual-luciferase reporter gene assay was conducted to verify the target binding relationship between miR-144-3p and E2F8.The regulatory mecha-nisms of miR-144-3p and E2F8 in LUAD were explored by real-time quantitative PCR(qRT-PCR),Western-blot,Cell Counting Kit-8(CCK-8)assay,colony formation assay,invasion assay and flow cytometry.The re-sults showed that miR-144-3p expression was significantly low in LUAD tissues and cells,while E2F8 ex-pression was significantly high.There was a targeting relationship between miR-144-3p and E2F8.Overex-pression of miR-144-3p or knockdown of E2F8 inhibited the proliferation and invasion of LUAD cells and arrested the cell cycle at G1 phase.In summary,miR-144-3p could inhibit the proliferation and invasion of LUAD cells and arrest the cell cycle at G1 phase by negatively regulating E2F8 expression,thereby inhibi-ting the malignant progression of LUAD cells.The results may be helpful for developing new methods in lung cancer detection and treatment.关键词
miR-144-3p/E2F转录因子 8(E2F8)/肺腺癌(LUAD)/增殖/侵袭Key words
miR-144-3p/E2F transcription factor 8(E2F8)/lung adenocarcinoma(LUAD)/proliferation/in-vasion分类
生物科学引用本文复制引用
宋世伟,李景武,刘晓辉..miR-144-3p靶向E2F8抑制肺腺癌细胞的增殖和侵袭并阻滞细胞周期[J].生命科学研究,2023,27(6):479-487,9.
