中国生态农业学报（中英文）2024，Vol.32Issue(1)：15-29,15.DOI:10.12357/cjea.20230267
抗虫转基因水稻及其杂交水稻对土壤微生物群落多样性与组成的影响
Effect of insect-resistant transgenic rice and its hybrid combination rice on diversity and composition of soil microbial community
摘要
Abstract
Microorganisms drive the soil material cycle and evolution of fertility,and their community composition is related to the stability and sustainability of the soil microecosystem.The study of changes in the soil microbial community in insect-resistant trans-genic rice is an important part of environmental safety assessments.Based on high-throughput sequencing of the bacterial 16S rRNA and fungal ITS genes,this study analyzed the differences in diversity and composition of microbial communities in paddy soils with insect-resistant transgenic rice'MFB',insect-resistant transgenic hybrid rice'Minfeng A/MFB''Tianfeng A/MFB'or'Gufeng A/MFB'and non-transgenic conventional rice'Minhui 3301'or hybrid rice'Tianyouhuazhan'.The results showed that,compared with non-transgenic conventional rice'Minhui 3301'or hybrid rice'Tianyouhuazhan',both insect-resistant transgenic rice'MFB'and transgenic hybrid rice'Minfeng A/MFB''Tianfeng A/MFB'or'Gufeng A/MFB'could significantly increase the yield(P＜0.05).At the same time,high-throughput sequencing showed that the α-diversity indexes of Chao1,Observed_species,and Shannon of bac-terial or fungal communities in paddy soil with'MFB'were higher than those in soil with'Minhui 3301'at all stage except for fungal communities at rice maturation stage,and the differences at rice maturation or tillering stage are significant(P＜0.05).At the heading stage of rice,the values of α-diversity indexes of Shannon of bacterial or fungal communities in paddy soils with the insect-resistant transgenic hybrid rice'Minfeng A/MFB''Tianfeng A/MFB'or'Gufeng A/MFB'were between those of soils with'MFB'and'Tianyouhuazhan'.The results of β-diversity analysis of bacterial or fungal communities showed that there were no significant differ-ences in the composition of microbial communities in paddy soils with different varieties of rice in this field experiment.However,the relative abundance of Proteobacteria,which showed the highest abundance of bacteria,in paddy soil with'MFB'increased com-pared with that of'Minhui 3301',and reached the significance level at the tillering and maturation stage of rice(P＜0.05).In contrast,the relative abundance of Ascomycota,which showed the highest abundance of fungi,was reduced in paddy soil with'MFB'and reached the significance level at the tillering and heading stages of rice(P＜0.05).At the heading stage of rice,the relative abundances of Proteobacteria and Ascomycota in paddy soils with'Minfeng A/MFB''Tianfeng A/MFB'or'Gufeng A/MFB'were between those of soils with'MFB'and'Tianyouhuazhan'.According to the functional prediction of microbial communities,the differences in func-tional composition of bacterial communities in paddy soil between soils with'MFB'and'Minhui 3301'gradually increased with rice growth.In summary,insect-resistant transgenic rice and its transgenic hybrid rice increased the diversity of soil bacterial and fungal communities and changed the relative abundance of major bacterial and fungal species with increased yield,but did not have signific-ant effects on the community and functional composition of bacteria or fungi.关键词
抗虫转基因水稻/细菌群落/真菌群落/高通量测序Key words
Insect-resistant transgenic rice/Bacterial community/Fungal community/High throughput sequencing分类
农业科技引用本文复制引用
宋亚娜,陈在杰,林艳,胡太蛟,吴明基,王锋..抗虫转基因水稻及其杂交水稻对土壤微生物群落多样性与组成的影响[J].中国生态农业学报（中英文）,2024,32(1):15-29,15.基金项目
福建省公益类科研院所专项(2021R1027002)、福建省政府与中国农业科学院合作农业优质发展和超越"5511"合作创新项目(XTCXGC2021002)和福建省科技重大专项(2020NZ08017)资助 This study was supported by Fujian Provincial Public Welfare Research Institute Special Project(2021R1027002),The'5511'Collaborative Innova-tion Project for High-quality Development and Surpasses of Agriculture between Government of Fujian Province and Chinese Academy of Agricultur-al Sciences(XTCXGC2021002),and Fujian Province Science and Technology Major Project(2020NZ08017). （2021R1027002）
