生物加工过程2024，Vol.22Issue(1)：17-24,8.DOI:10.3969/j.issn.1672-3678.2024.01.003
假单胞菌Rs-198的IAA合成途径分析
Analysis of indole acetic acid(IAA)synthesis pathway in Pseudomonas putida Rs-198
摘要
Abstract
The indole acetic acid(IAA)synthesis pathway in Pseudomonas putida Rs-198 was thoroughly investigated through the experiments with various additives to the nutrient medium(NA).Upon the addition of tryptophan and its metabolic intermediates to the nutrient medium,the growth of P.putida Rs-198 and ultimate IAA production were evaluated.As a result,the tryptophan additions at different concentrations could significantly improve the IAA production by 2 to 5 times although the Rs-198 cell concentrations were barely changed.Notably,Rs-198 cells grew well under 50 mg/L tryptophan condition and the maximum IAA production in this case reached 55.52 mg/L at 96 h,which was 3.58 times of the control.Further analysis of the fermentation broth with high performance liquid chromatography(HPLC)showed the contents of tryptamine and indole pyruvic acid gradually decreased during the IAA production,while indole acetamide and indole acetonitrile remained at similar levels,suggesting that tryptamine(TAM)and indole pyruvate(IPyA)were involved in the main pathway for the synthesis of IAA in P.putida Rs-198.Additionally,the production of IAA by P.putida Rs-198 was confirmed by a pot experiment,in which significant improvement was observed not only for the seeding rate of pepper but also for the continuous pepper growth.关键词
假单胞菌/吲哚-3-乙酸/色氨酸/合成途径/促生Key words
Pseudomonas putida/indole-3-acetic acid/tryptophan/synthesis pathway/growth promoting分类
生物科学引用本文复制引用
王立娟,杨义琳,高慧鑫,张延妍,何艳慧,武占省,卢华丹..假单胞菌Rs-198的IAA合成途径分析[J].生物加工过程,2024,22(1):17-24,8.基金项目
陕西省教育厅一般专项科研计划(21JK0643) （21JK0643）
西安工程大学大学生创新创业训练项目(S202110709126) （S202110709126）
