生物加工过程2024，Vol.22Issue(1)：25-32,8.DOI:10.3969/j.issn.1672-3678.2024.01.004
贻贝足蛋白fp-5在大肠杆菌中的表达、修饰与功能分析
Expression,modification and functional analysis of mussel foot protein fp-5 in Escherichia coli
摘要
Abstract
The co-expression of Mytilus galloprovincialis foot protein fp-5 and tyrosinase from different bacterial sources in E.coli could produce the modified mussel foot protein with improved in vivo performance.Through sequence analysis and codon optimization,both single-vector and dual-vector co-expression systems were constructed with tyrosinase and mussel foot protein genes,and the subsequent expression proved to be successful in E.coli.The purified protein was analyzed by NBT/Gly staining test and acid-borate differential spectroscopy,showing a wide range of hydroxylation levels in the resultant fp-5.The co-expression of fp-5 and tyrosinase derived from Verrucomicrobium spinosum(TyrVs)reached a concentration of 18.6 mg/L,along with 55.2％modification rate.Moreover,the 5-Vs from such co-expression showed much higher adhesion force(4.6 times)than the unmodified fp-5.Due to the excellent adsorption and adhesion,the 5-Vs modified in vivo by TyrVs will provide a potential bio-glue material for biomedicine and other fields.关键词
贻贝足蛋白/酪氨酸酶/共表达/大肠杆菌/体内修饰Key words
mussel foot protein/tyrosinase/co-expression/Escherichia coli/modification in vivo分类
生物科学引用本文复制引用
姚林,谢紫莎,王瑞,张鲁嘉,李莎..贻贝足蛋白fp-5在大肠杆菌中的表达、修饰与功能分析[J].生物加工过程,2024,22(1):25-32,8.基金项目
国家重点研发计划(2019YFA0905203) （2019YFA0905203）
国家自然科学基金(22178177) （22178177）
江苏省先进生物制造创新中心项目自主课题(XTD2201) （XTD2201）
