生物加工过程2024，Vol.22Issue(1)：33-41,9.DOI:10.3969/j.issn.1672-3678.2024.01.005
阴离子交换树脂固定化核酸酶P1的研究
Immobilization of nuclease P1 by anion exchange resin
摘要
Abstract
The market demand for 5'-nucleotides and their derivatives keeps increasing because of their high nutritional and health care value and broad medical application.Nuclease P1 is currently the key enzyme in the industrial production of 5'-nucleotides,but the free enzyme form still suffers from limitations such as difficulty in reuse and excessive consumption.In order to solve the above problems and improve its utilization efficiency,different types of resins were evaluated for the immobilization of nuclease P1 in this study.Based on the adsorption amount of nuclease P1 and the enzymatic activity of the immobilized enzyme,the anion exchange resin 1(AER1)offered the best immobilization effects.The optimal immobilization conditions were described as follows:the amount of enzyme was 3 644.7 U/ml(2.2 mL),the mass ratio of glutaraldehyde was 0.25％,the cross-linking time was 1.5 h,the immobilization time was 10.0 h,and the pH was 5.1.The specific activity of such immobilized nuclease P1 was 47 980.95 U/g.More importantly,the immobilized enzyme not only displayed the improvement of thermal stability to a certain extent,but also demonstrated much better acid-base stability.Compared with the free enzyme,the immobilized nuclease P1 also showed excellent storage stability,with 51.8％retention of its enzymatic activity after it was stored at 4 ℃ for 6 weeks.关键词
核酸酶P1/树脂/固定化酶/交联Key words
nuclease P1/resin/immobilized enzyme/cross-linked分类
生物科学引用本文复制引用
王天赐,徐康,侯亚利,杨忠华..阴离子交换树脂固定化核酸酶P1的研究[J].生物加工过程,2024,22(1):33-41,9.基金项目
国家自然科学基金(21376184) （21376184）
湖北省教育厅科研基金重点项目(d20121108) （d20121108）
