生物加工过程2024，Vol.22Issue(1)：42-49,8.DOI:10.3969/j.issn.1672-3678.2024.01.006
一株产表面活性剂菌株的筛选、培养条件优化及产物性质
Screening,optimization of culture conditions and characteristics of its product of a surfactant producing strain
摘要
Abstract
A bacterium capable of producing surfactant was screened from the sediment of sewage plant in the western suburb of Changchun.The colony of the strain is small and milky white.The individual shape is round under the microscope,and is gram-negative.The results of 16S rDNA sequence showed that the strain belonged to Pseudomonas sp.and was 100％similar to Pseudomonas libanensis(KT767978),was named Pseudomonas libanensis PbA.Product of 0.54 g/L could be synthesized by culturing the strain using glucose as carbon source and KNO3 as nitrogen source with the inoculation amount was 5％mass fraction at 27 ℃,pH 7 and cultured for 3 days.Analysis results showed that the synthetic product from the strain was glycolipid anionic surfactant by charged result,TLC analysis,and sugar content determination.The emulsification index and the diameter of oil drain ring of the surfactant with a concentration of 0.04 mg/mL was 60.3％and 5.6 cm,respectively.The surface tension of the surfactant decreased from 65.3 mN/mi(concentration 0.4 g/L)to 54.1 mN/m(concentration＞106 mg/L)with the increase of concentration,and the critical micelle concentration(CMC)was 106 mg/L.The basic data are provided for enriching the surfactant praducing bacteria,opening the fermentation production process and the application of surfactants.关键词
菌种筛选/假单胞菌Pseudomonas libanensis PbA/糖脂类生物表面活性剂/产物性质Key words
strain identification/Pseudomonas libanensis PbA/glycolipid biosurfactants/product properties分类
化学化工引用本文复制引用
肖燕然,周爽姿,李爽,张思宇,李雪,孟珈同,付永平,韩雪容..一株产表面活性剂菌株的筛选、培养条件优化及产物性质[J].生物加工过程,2024,22(1):42-49,8.基金项目
国家自然科学面上项目(31971252) （31971252）
吉林省发展改革委员会项目(产业技术与研究开发项目)(2020C028-3) （产业技术与研究开发项目）
