生物加工过程2024，Vol.22Issue(1)：106-112,7.DOI:10.3969/j.issn.1672-3678.2024.01.014
快速样品前处理技术-超高效液相色谱串联质谱法测定粮谷中7种真菌毒素
Determination of seven mycotoxins ingrains using QuEChERS and ultra-performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
QuEChER,a fast and fast sample preprocessing technology(quick、easy、cheap、effective、rugged、safe),was applied to determine the contents of seven mycotoxin residues in grains through the combination with UPLC-MS/MS.After the samples were extracted with acetonitrile-water-formic acid(84:15.5:0.5)solution and purified by N-propyl ethylene diamine(PSA),UPLC-MS/MS was used for the detection.The excellent linearity was observed for the seven mycotoxins in the concentration range of 0.15-250 μg/L,with the correlation coefficients(R2)higher than 0.99.In addition,the detection and quantification limits of our method were 0.02-13.22 μg/L and 0.08-39.55 μg/L,respectively.The average spiked recoveries were 82.44％-103.12％,with RSD less than 10％.When this method was utilized to measure the seven mycotoxins in rice,beans,peanuts and other cereal crops,the spiked recoveries turned out to be greater than 82％,further demonstrating the general applicability of our analytical method.关键词
QuEChERS/高效液相色谱串联质谱法/粮谷/真菌毒素/食品安全Key words
QuEChERS/UPLC-MS/MS/grains/mycotoxins/food safely分类
轻工纺织引用本文复制引用
殷锡峰,梁红芳,张文文,吴琴燕..快速样品前处理技术-超高效液相色谱串联质谱法测定粮谷中7种真菌毒素[J].生物加工过程,2024,22(1):106-112,7.基金项目
句容市重点研发计划(ZA42114) （ZA42114）
