山西大学学报（自然科学版）2024，Vol.47Issue(1)：238-247,10.DOI:10.13451/j.sxu.ns.2023097
微残清颗粒逆转白血病干细胞耐药的机制研究
Study on the Mechanism of Reversing Drug Resistance of Leukemia Stem Cells by WeiCanQing Granule
摘要
Abstract
This study explores the mechanism of action of WeiCanQing granule in reversing the resistance of leukemia stem cells to daunorubicin(DNR).Using the method of intervening CD34+CD38-KG1a leukemia stem cells with rabbit serum containing Wei-CanQing granule,CD34+CD38-KG1a cells sorted by immunomagnetic beads were divided into control group,DNR group,WeiCan-Qing granule group,and WeiCanQing granule+DNR group.Phosphatase and tensin homolog(PTEN)expression was silenced using siRNA method,and cell proliferation rate,cell cycle/apoptosis ratio,and Intracellular phosphatidylinositol kinase(PI3K)/phosphory-lated protein kinase B(p-AKT)/phosphorylated rapamycin target protein(p-mTOR)mRNA and protein levels were examined.The results showed that the combination of WeiCanQing granule containing serum and DNR significantly increased the proliferation inhi-bition rate,G2/M phase ratio,total apoptosis,and early apoptosis rate of CD34+CD38-KG1a cells.At the same time,the drug con-taining serum of WeiCanQing granule synergistically enhanced the expression of PTEN mRNA and protein,and reduces the expres-sion of PI3K,p-AKT,p-mTOR mRNA and protein.The above results suggest that WeiCanQing granule have the effect of reversing LSCs'resistance to DNR,and the mechanism of action may be related to negative regulation of PTEN on the PI3K/AKT/mTOR pathway.关键词
微残清颗粒/白血病干细胞/耐药/PTEN/PI3K/AKT/mTOR通路Key words
WeiCanQing granule/leukemia stem cells/drug resistance/PTEN/PI3K/AKT/mTOR分类
医药卫生引用本文复制引用
闫理想,姜静,冯全管,杨向东,何敬,杨曦,李德冠,史哲新..微残清颗粒逆转白血病干细胞耐药的机制研究[J].山西大学学报（自然科学版）,2024,47(1):238-247,10.基金项目
天津中医药大学第一附属医院"拓新工程"基金项目(院2020057) （院2020057）
天津市教委科研计划项目(2021KJ145) （2021KJ145）
天津市科技计划项目(21JCQNJC01210) （21JCQNJC01210）
