陕西林业科技2023，Vol.51Issue(4)：1-4,10,5.DOI:10.3969/j.issn.1001-2117.2023.04.001
紫芽茶脱毒苗组织培养再生体系的建立
Regeneration System of Virus—free Seedlings of Camellia sinensis var.kitamura in Different Tissue Culture
摘要
Abstract
The young leaves,petioles and stem segments of Camellia sinensis var.kitamura were used as explants to compare the effects of different concentrations of hormone ratios of NAA,6—BA,KT,IBA and 2,4—D on the induction and differentiation of different tissues.The results showed that un-der the conditions of 75％alcohhol and 0.1％HgCl2 sterilization and 1/2 MS culture,1.0 mg·L-1 2,4—D and 0.5 mg·L-1 KT combined treatment of callus growth was the best,the survival rate was a-bout 80％.With the young leaves,petioles and stem segments as the explants of Camellia sinensis var.kitamura,different tissue culture and regeneration systems of Camellia sinensis var.kitamura could be established through appropriate hormone treatment.关键词
紫芽茶/外植体/愈伤组织/再生体系Key words
Camellia sinensis var.kitamura/explant/callus/regeneration system分类
农业科技引用本文复制引用
王赟,侯楠,景慧,赵国平,魏树和,贾根良,代惠萍..紫芽茶脱毒苗组织培养再生体系的建立[J].陕西林业科技,2023,51(4):1-4,10,5.基金项目
秦巴生物资源与生态环境国培重点实验室开放课题(SLGPT2019KF04-02) （SLGPT2019KF04-02）
国家"十三五"重点研发计划课题(2018YFC1800501) （2018YFC1800501）
科技部项目(G2022040018L). （G2022040018L）
