现代检验医学杂志2024，Vol.39Issue(1)：16-22,7.DOI:10.3969/j.issn.1671-7414.2024.01.004
生物信息学方法筛选IL-3和IL-3+SCF诱导的小鼠骨髓来源肥大细胞的差异表达基因及相关信号通路分析
Screening of IL-3 and IL-3+SCF Induce Differentially Expressed Genes and Signaling Pathways in Bone Marrow-derived Mast Cells Based on Bioinformatics
摘要
Abstract
Objective To identify the differentially expressed genes and pathways of bone marrow-derived mast cells(BMMCs)of mice induced by IL-3 and IL-3+stem cell factor(SCF)using bioinformatics analysis,which may provide a foundation for in vitro culture and functional study of mast cells(MC).Methods The matrix data of GSE35332 dataset in IL-3 and IL-3+SCF induced BMMCs was downloaded from the GEO database,and the R software was applied to screen differentially expressed genes(DEGs).The gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis of EDGs were performed based on the online tool DAVID database.The protein interaction network was constructed by STRING database and hub genes were screened through MCODE plugin of the Cytoscape software.Results The GSE35332 data set was analyzed by R software,and 1 339 DEGs were screened,including 723 up-regulated genes and 616 down-regulated genes.A total of 6 hub genes were screened through the MCODE plugin of Cytoscape software,namely Psmd8,Psmd6,Psmd14,Psmc4,Psma6 and Psma3.GO and KEGG analysis showed that the hub genes were concentrated in proteolysis,antigen processing and presentation of exogenous peptide antigen via MHC class I,proteasome-mediated ubiquitin-dependent protein catabolism process,and Epstein-Barr virus infection.Conclusion This study found that there were significant differences in BMMCs gene expression profiles in mice induced by two modes and 6 hub genes participated in ubiquitin-dependent protein decomposition process through bioinformatics based on the GEO database,providing help for further research on MC vitro culture and function.关键词
肥大细胞/干细胞因子/差异表达基因/生物信息学Key words
mast cells/stem cell factor/differentially expressed genes/bioinformatics分类
医药卫生引用本文复制引用
曹君,金婕妤,张胜,乔龙威,梁玉婷..生物信息学方法筛选IL-3和IL-3+SCF诱导的小鼠骨髓来源肥大细胞的差异表达基因及相关信号通路分析[J].现代检验医学杂志,2024,39(1):16-22,7.基金项目
国家自然科学基金(81901632):Mc-Exo通过DCN胞间转移调控气道平滑肌细胞表型转化在哮喘中的作用及研究机制 （81901632）
(82001576):基于孕妇cfDNA特征分析在自发性早产启动中的作用及机制研究. （82001576）
