现代检验医学杂志2024，Vol.39Issue(1)：95-99,5.DOI:10.3969/j.issn.1671-7414.2024.01.017
特发性矮小症患儿血清Vaspin和SFRP5水平表达及其诊断价值研究
Expression of Serum Vaspin and SFRP5 in Children with Idiopathic Nanosomia and Their Diagnostic Value
摘要
Abstract
Objective To explore the expression level of visceral adipose tissue-derived serine protease inhibitor(Vaspin)and secreted frizzled-related protein5(SFRP5)in the serum of children with idiopathic short stature(ISS)and its diagnostic value.Methods 70 children with ISS diagnosed in the First Hospital of Zhangjiakou from December 2021 to February 2023 were selected as the disease group,while 72 healthy volunteer children who underwent physical examination were collected as the control group.Immunoluminescence was applied to detect the expression level of VASPIN,Enzyme-linked immunosorbent assay(ELISA)was applied to detect the expression level of SFRP5 the clinical data of children in two groups were analyzed.Receiver operating characteristic(ROC)curve was applied to analyze the diagnostic value of serum Vaspin and SFRP5 for ISS,multivariate Logistic regression was used to analyze the influencing factors of ISS.Results Compared with the control group,the serum Vaspin level in the disease group was obviously increased(2.89±0.92 ng/ml vs 1.81±0.42 ng/ml),while the SFRP5 level was obviously reduced(10.22±2.84 pg/ml vs 13.21±3.53 pg/ml),the differences were statistically significant(t=9.040,5.552,all P＜0.05).The weight,height,body mass index(BMI)and proportion of sexual development stage II～V of children in the disease group were obviously lower than those in the control group,and the differences were statistically significant(t=7.687,6.330,5.559,7.024,all P＜0.05).The area under ROC curve showed that the AUC of Vaspin and SFRP5 and their combined detection in the diagnosis of ISS were 0.768,0.849 and 0.925,respectively,the combined diagnosis efficacy of Vaspin and SFRP5 was better than that of serum Vaspin and SFRP5 alone(Z =3.829,P＜0.001;Z =2.141,P=0.032).Multivariate Logistic regression analysis showed that BMI(OR=0.508,95%CI:0.260～0.991),Vaspin(OR=3.458,95%CI:1.125～10.631)and SFRP5(OR=0.378,95%CI:0.153～0.935)were the influencing factors for ISS(all P＜0.05).Conclusion The expression level of Vaspin in the serum of children with ISS is obviously increased,while the expression level of SFRP5 is obviously reduced.The two are influencing factors of ISS,and the combined detection of their expression levels has certain value in the diagnosis of ISS.关键词
特发性矮小症/内脏脂肪特异性丝氨酸蛋白酶抑制剂/分泌型卷曲相关蛋白5Key words
idiopathic short stature/visceral adipose tissue-derived serine protease inhibitor/secreted frizzled-related protein 5分类
医药卫生引用本文复制引用
吴迎爽,薛向东,杜志辉,王利红,程渊博,牛晓庆,王大伟..特发性矮小症患儿血清Vaspin和SFRP5水平表达及其诊断价值研究[J].现代检验医学杂志,2024,39(1):95-99,5.基金项目
张家口市重点研发项目(编号1921085D):浆细胞瘤多样性异位基因1在小儿激素敏感型和抵抗型支气管哮喘中的表达及作用机制研究. （编号1921085D）
