现代检验医学杂志2024，Vol.39Issue(1)：126-130,5.DOI:10.3969/j.issn.1671-7414.2024.01.023
基于流式量子点微球技术的甲乙型流感病毒抗原检测方法的建立和初步应用分析
Establishment and Preliminary Application Analysis of A Multiplex Detection Method for Influenza A and B Virus Antigen Based on Quantum Dot-encoded Microsphere Flow Cytometry Technology
摘要
Abstract
Objective To establish a multiplex assay method for the simultaneous detection of FluA and FluB virus(IBV)antigen based on the flow cytometry(FCM)quantum dot-encoded bead technologies,laying the foundation for the assay of multiple respiratory virus biomarkers.Methods Coupling was performed for FluA and FluB nucleoprotein(NP)monoclonal antibodies using self-made quantum dot-encoded beads,separately.FCM was used to detect known concentrations of FluA and FluB antigens separately and simultaneously,optimize the detection conditions,and establish a joint detection method for FluA and FluB antigens.Compared with the quantitative real-time PCR(qPCR)method,clinical samples were used to evaluate the clinical performance of this joint detection method.Results The joint detection method for FluA and FluB antigens was established,with detection limits of 26.1 pg/ml and 10.7 pg/ml,respectively,and measurement ranges of 15.3～250 000 pg/ml.The joint detection method for clinical sample evaluation was well correlated with the qPCR,with a positive coincidence rate of 57.4%,a negative coincidence rate of 100%,and a total coincidence rate of 71.6%.In addition,the joint detection method was superior to colloidal gold immunochromatographic strip assay commonly used in clinical practice(positive coincidence rate of 56.49%,negative coincidence rate of 99.75%).Conclusion The FCM quantum dot-encoded bead multiplex assay can be used for the joint detection of FluA and FluB antigens,which have a high sensitivity,good specificity and wide detection range.It may lay a good foundation for the multiplex detection of common respiratory viruses,and has clinical application prospects.关键词
甲型流感病毒/乙型流感病毒/量子点编码微球/液相蛋白芯片Key words
FluA/FluB/quantum dot-encoded beads/liquid protein chip分类
医药卫生引用本文复制引用
夏成静,李宝花,郭燕妳,周小合,张润玲,牛英波..基于流式量子点微球技术的甲乙型流感病毒抗原检测方法的建立和初步应用分析[J].现代检验医学杂志,2024,39(1):126-130,5.基金项目
深圳光明区软科学研究项目(项目批号2021R01097). （项目批号2021R01097）
