实用医学杂志2024，Vol.40Issue(1)：25-31,7.DOI:10.3969/j.issn.1006-5725.2024.01.005
Kruppel样因子14介导的JAK-STAT信号通路对非小细胞肺癌预后的影响
Effect of the KLF14-mediated JAK-STAT signaling pathway on prognosis of lung cancer
摘要
Abstract
Objective To investigate the influence of the Janus kinase-signal transducer and transcription activator(JAK-STAT)signaling pathway mediated by Kruppel-like factor 14(KLF14)on the prognosis of non-small cell lung cancer(NSCLC).Methods From January 2018 to September 2019,NSCLC tissues from 80 patients and malignancy-free paracancerous tissues from 25 patients were collected.Medical follow-up ended in April 2023.Immunohistochemistry was used to detect the expression of KLF14 in tissues,and the patients were divided into a high-expression group and a low-expression group according to the median level of KLF14 expression.Over-expres-sion or knock-down of KLF14 and JAK1 was achieved by transfection of KLF14 and JAK1 overexpression plasmid in A549 cells and transfection of KLF14 and JAK1 specific short hairpin RNA(shKLF14 and shJAK1)in HCC827 cells.The proliferation activity of cells was analyzed by cell clone formation test.Transwell analyzed the migration and invasion of cells.Results As compared with the normal paracancerous tissues,the expression of KLF14 in NSCLC tissue decreased(P<0.001).The low expression of KLF14 was significantly correlated with tumor diameter of>3 cm,lymph node metastasis and clinical stage Ⅲ(P<0.05).There was a significant difference in the overall survival rate between the high KLF14 expression group and the low KLF14 expression group,and the patients with low KLF14 expression had poor prognosis(P = 0.039).After overexpression of KLF14,the proliferation ability of A549 cells and the number of migration and invasion of these cells decreased significantly(P<0.05);while after knock-down of KLF14,the proliferation ability of HCC827 cells and the number of migration,and invasion of these cells increased significantly(P<0.05).As compared with Vector + KLF14 group,the number of colonies,migration and invasion of A549 cells in JAK1 + KLF14 group increased significantly(P<0.05).As compared with shNC + shKLF14 group,the number of colonies,migration and invasion of HCC827 cells in shJAK1 + shKLF14 group decreased significantly(P<0.05).Conclusions Low expression of KLF14 is associated with poor overall survival in NSCLC patients.Up-regulation of KLF14 significantly inhibits the proliferation and metastasis of lung cancer cells in vitro,and its mechanism may be related to inhibition of the JAK-STAT signaling pathway.关键词
Kruppel样因子14/Janus激酶-信号转导子与转录激活子/非小细胞肺癌/预后Key words
kruppel-like factor 14/janus kinase-signal transducer and transcriptional activator/non-small cell lung cancer/prognosis分类
医药卫生引用本文复制引用
王鹏,姚苏梅,吕学东,陈金亮..Kruppel样因子14介导的JAK-STAT信号通路对非小细胞肺癌预后的影响[J].实用医学杂志,2024,40(1):25-31,7.基金项目
江苏省卫生健康委员会科研项目(编号:M20200096) （编号:M20200096）
南通市科技计划项目(编号:JCZ18130) （编号:JCZ18130）
