实用医学杂志2024，Vol.40Issue(1)：65-71,7.DOI:10.3969/j.issn.1006-5725.2024.01.012
同种异体移植物炎性因子-1在糖尿病睾丸大鼠模型中的表达及意义
Expression of allograft inflammatory factor-1 in the testicular model of diabetes mellitus rats
摘要
Abstract
Objective To investigate the expression of allograft inflammatory factor-1(AIF-1)in the testicular model of diabetes mellitus(DM)rats as well as its significance.Methods The rat model of DM testis(DMT)was established,which were randomly divided into the DM testis 4-week group(DMT4W),DM testis s 8-week group(DMT8W)and the DM testis 12-week group(DMT12W).The normal control group(NC group)was randomly divided into three subgroups:NC 4-week(NC4W),NC 8-week(NC8W)and NC 12-week(NC12W).The morphologic changes of testis in the different groups was detected by histopathology.The expression of AIF-1 protein was detected by immunohistochemistry.The expression of AIF-1 and NF-κB p65 protein was observed by immunofluorescence.Results The histopathological results suggested that the numbers of spermatogenic cells,sertoli cells,interstitial cells and sperms in the DMT group were significantly decreased,as compared with the NC group.The immunohistochemistry results showed that the expression of AIF-1 protein was significantly increased in the DMT group,as compared with the NC group(P<0.05).The intensity of AIF-1 and NF-κB p65 in the DMT group was significantly increased by immunofluorescence,as compared with the NC group.Conclusion The over expression of AIF-1 protein in DMT tissue suggests that it may play an important role in the pathological process of DMT and may become a new therapeutic target and diagnostic marker in the future.关键词
同种异体移植物炎性因子-1/糖尿病睾丸/大鼠/模型/意义Key words
allograft inflammatory factor-1/diabetes mellitus testis/rat/model/significance分类
医药卫生引用本文复制引用
李德朝,张明津,蓝一笔,马春雷,付伟金..同种异体移植物炎性因子-1在糖尿病睾丸大鼠模型中的表达及意义[J].实用医学杂志,2024,40(1):65-71,7.基金项目
广西泌尿系统疾病临床医学研究中心(编号:2020AC03006) （编号:2020AC03006）
广西科技基地和人才专项(编号:2019AC17009) （编号:2019AC17009）
