实用医学杂志2024，Vol.40Issue(1)：91-96,6.DOI:10.3969/j.issn.1006-5725.2024.01.016
江西省耐多药结核分枝杆菌链霉素基因突变特征
Study on the mutation characteristics of streptomycin genes of Multidrug-Resistant Mycobacterium Tuber-culosis in Jiangxi Province
摘要
Abstract
Objective To understand the molecular characteristics of Streptomycin(SM)resistance in multidrug-resistant tuberculosis(MDR-TB)in Jiangxi Province,and to explore the relationship between SM resistant genes(rpsL,rrs and gidB)mutations and SM resistant phenotypes in Beijing genotype TB.Methods 106 non-replicated MDR-TB isolates were collected from Gaoxin Branch of The First Affiliated Hospital of Nanchang University and Jiangxi Provincial Chest Hospital from January to December 2021,and tested for drug-resistance phenotypes,whether they were Beijing genotype or not and the characteristics of rpsL,rrs and gidB gene mutations.Chi-square test was performed to determine whether rpsL,rrs and gidB mutations were related to genotypes and drug-resistance phenotypes.Results Among 106 cases of MDR-TB,76 cases were resistant to SM.A total of 58 cases had rpsL 43A>G mutation,8 cases had 88A>G mutation,5 cases had rrs mutation,and 3 cases had gidB mutation.Statistical analysis showed that the coincidence rate of gene mutation and phenotypic drug-resistance detection was 89.6%,and the specificity and sensitivity were 86.7%and 90.8%,respectively.The isolated rate of Beijing genotype TB was 88.7%,and the drug-resistant gene mutations were mainly concentrated in rpsL and rrs,while the drug-resistant mutations of non-Beijing genotype were mainly concentrated in gidB;in addition,Beijing genotype bacteria were more prone to gene mutations(P = 0.013),but there was no difference in phenotypic drug-resistance.Conclusions Mutations in rpsL,rrs,and gidB genes have a good coincidence rate with phenotypic drug-resistance,and molecular biology can be used to detect directly drug-resistance genes to predict bacterial resistance;TB genotypes are strongly associated with streptomycin resistance characteristics.关键词
耐多药结核菌/链霉素耐药/北京基因型/江西省Key words
multidrug-resistant tuberculosis/streptomycin resistance/beijing genotype/Jiangxi province分类
医药卫生引用本文复制引用
于圣铭,夏良华,詹佳欢,刘思琦,王伟,晏亮,陈开森..江西省耐多药结核分枝杆菌链霉素基因突变特征[J].实用医学杂志,2024,40(1):91-96,6.基金项目
国家自然科学基金项目(编号:82060611) （编号:82060611）
江西省科技合作一般项目(编号:20212BDH81017) （编号:20212BDH81017）
