土壤学报2023，Vol.60Issue(6)：1799-1809,11.DOI:10.11766/trxb202203180120
长期耕作对农田黑土几丁质降解菌群及酶活性的影响
Effects of Long-term Different Tillage Practices on Chitin Degrading Microbial Communities and Chitinase Activity in Farmland Black Soil
摘要
Abstract
[Objective]This study aimed to illustrate the impacts of different tillage practices on chitin degrading microbial communities and chitinase activity in farmland black soil,and to explore the main environmental factors in driving a change in chitin degrading microbial communities and chitinase activity.[Method]Based on the long-term positioning platform of different tillage practices in black soil and the combination of fluorescence quantification and high throughput sequencing technology,this research intends to study the effects of long-term different tillage practices(Conventional tillage,No-tillage,Sub-soiling tillage,Moldboard plowing tillage)on chitin degrading bacteria harboring chiA gene abundance,microbial communities and chitinase activity in 0-40 cm soil layers.[Result]Results showed that no-tillage increased chiA gene abundance in the 0-20 cm soil layer.Lower chiA gene abundance,alpha diversity and proteobacteria relative abundance,and higher actinobacteria relative abundance in the 20-40 cm soil layer of no-tillage than other tillage practices.The abundance of chiA gene and microbial community structure was significantly affected by soil pH,mean weight diameter and nutrients.Compared with conventional tillage,the chitinase activity increased in 0-20 cm soil layer under no-tillage,and the chitinase activity was enhanced in 0-40 cm soil layer under sub-soiling tillage and moldboard plowing tillage.The simulation result of the structural equation modeling showed that chitinase activity was directly affected by the tillage practice,soil depth,mean weight diameter,organic carbon,total nitrogen,total phosphorus,chiA gene abundance,and actinobacteria relative abundance.[Conclusion]These results provide a theoretical basis for understanding the effect of different tillage practices on soil chitin degradation in black soil areas.关键词
耕作措施/几丁质降解菌/chiA基因/几丁质酶活性/黑土Key words
Tillage practices/Chitin degrading microorganism/chiA gene/Chitinase activity/Black soil分类
农业科技引用本文复制引用
隋鹏祥,罗洋,李瑞平,郑洪兵,袁野,郑金玉,王浩,刘武仁..长期耕作对农田黑土几丁质降解菌群及酶活性的影响[J].土壤学报,2023,60(6):1799-1809,11.基金项目
中国博士后科学基金资助项目(2021M693907)和吉林省农业科技创新工程项目(CXGC2020RCG010,CXGC2021RCB003)资助Supported by the China Postdoctoral Science Foundation(No.2021M693907),and the Jilin Province Agricultural Science and Technology Innovation Project(Nos.CXGC2020RCG010,CXGC2021RCB003) （2021M693907）
