摘要
Abstract
Objective To explore the mechanism of human rhinovirus(HRV)-induced acute exacerbation of pediatric asthma and find the potential therapeutic targets by bioinformatics analysis.Methods The raw data of GSE30326 dataset were downloaded from Gene Expression Omnibus(http://www.ncbi.nlm.nih/gov/geo)and analyzed with R language to screen out the differentially expressed genes(DEGs)in HRV-induced acute exacerbation of pediatric asthma and clinical remission.Gene ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis were performed on these DEGs,and the protein-protein interaction(PPI)network was constructed to extract the core genes and functional modules.Results A total of 327 DEGs were screened out in the GSE30326 dataset,including 318 up-regulated genes and 9 down-regulated genes.GO analysis showed that the DEGs were enriched in biological processes such as antiviral defense response,regulation of innate immune response,type Ⅰinterferon signaling pathway,molecular functions such as single and double stranded RNA adhesion,G-protein coupled receptor binding,chemokine receptor binding,synthesis of 2',5'-oligoadenylate,and cellular components such as vesicles and external side of serosa.KEGG analysis showed that the DEGs were mainly enriched in NOD-like receptor signaling pathway,Toll-like receptor signaling pathway,RIG-I-like receptor signaling pathway,interaction between cytokine receptors,chemokine signaling pathway,antigen presenting,cell adhesion molecule,Jak-STAT signaling pathway and endocytosis.The PPI network constructed by DEGs encoding proteins was consisted of 185 nodes and 958 interaction lines,with the largest functional module consisting of 30 nodes and 373 interaction lines.GO analysis of the genes of these 30 nodes showed that the DEGs were mainly enriched in biological processes such as type Ⅰ interferon signaling pathway,antiviral defense response,and negative regulation of viral genome replication.The five nodes with high topology score in the PPI network,namely the core genes,were 2',5'-oligoadenylate synthetases 2(OAS2),interferon regulatory factor 7(IRF7),signal transduction and activator of transcription 1(STAT1),2',5'-oligoadenylate synthetases 3(OAS3)and 2',5'-oligoadenylate synthetase like protein(OASL).Conclusion NOD-like receptor signaling pathway,Toll-like receptor signaling pathway,RIG-I-like receptor signaling pathway are involved in the HRV-induced acute exacerbation of pediatric asthma.In addition,OAS2,IRF7,STAT1,OAS3 and OASL act on interferon or its downstream effector pathway,and may play an important role in the body's anti HRV infection,which are expected to be the new therapeutic targets.关键词
儿童哮喘/急性发作/人鼻病毒/生物信息学分析Key words
Pediatric asthma/Acute exacerbation/Human rhinovirus/Bioinformatics analysis分类
临床医学
