武警医学2024，Vol.35Issue(1)：25-29,5.
生长分化因子11激活PI3K/Akt信号途径促进糖尿病小鼠ADSCs体外矿化的研究
Promotion of GDF11 on osteoblast differentiation of ADSCs derived from diabetic mice by activating PI3K/Akt signaling pathway
摘要
Abstract
Objective To investigate the effects of growth differentiation factor 11(GDF11)on osteoblast differentiation of adipose derived stem cells(ADSCs)from diabetic mice and related signaling mechanism.Methods ADSCs from diabetic mice were i-solated and cultured.The effect of GDF11 on the proliferation of ADSCs was detected by flow cytometry.After cultured in osteogenic differentiation medium,ALP activity was detected,qPCR was used to evaluate the expression of osteoblast genes,and phosphorylation levels of PI3K and Akt were detected by western blot.Results Cell proliferation was less affected by GDF11 intervation,promoted ALP activity in a concentration-dependent but saturated manner,significantly upregulated mRNA expression of Runx2(2.41±0.19)vs.(1.00±0.11)],Osx[(1.41±0.21)vs.(1.00±0.10)]and ALP[(1.42±0.20)vs.(1.00±0.09)](P<0.05),and signifi-cantly increased phosphorylation levels of PI3K[(2.84±0.19)vs.(1.00±0.11)]and Akt[(4.58±0.20)vs.(1.00±0.19)](P<0.05).Moreover,the effects of GDF11 on promoting osteoblast differentiation of ADSCs from diabetic mice was partially weak-ened by the PI3K inhibitor LY294002.Conclusions GDF11 promotes osteoblast differentiation of ADSCs from diabetic mice,and its mechanism may be related with the activation of PI3K/Akt signaling pathway.关键词
生长分化因子11/PI3K/Akt信号通路/脂肪间充质干细胞/成骨分化Key words
growth differentiation factor 11(GDF11)/PI3K/Akt signaling pathway/ADSCs/osteoblast differentiation分类
医药卫生引用本文复制引用
龚闽,朱彪,李欣,梅美,党瑞杰,陈晔..生长分化因子11激活PI3K/Akt信号途径促进糖尿病小鼠ADSCs体外矿化的研究[J].武警医学,2024,35(1):25-29,5.基金项目
中国博士后科学基金(2019T120980) （2019T120980）
