弹性蛋白对前交叉韧带力学响应影响的弹性蛋白酶定量分析
Elastase quantitative analysis of elastin effect on mechanical response of anterior cruciate ligament
摘要
Abstract
BACKGROUND:The anterior cruciate ligament has unique nonlinear mechanical properties under a complex physiological loading environment.Elastin is an important contributor to the mechanical properties of the anterior cruciate ligament,but its mechanical response to the anterior cruciate ligament under axial tension is not clear. OBJECTIVE:To quantitatively analyze the effect of elastin on the tensile mechanical response of the anterior cruciate ligament. METHODS:Elastase solution and control buffer were prepared.The porcine anterior cruciate ligament samples were prepared into small-size samples and randomly soaked in 0,0.1,1.0,2.0,5.0,and 10.0 U/mL elastase solution for 6 hours,and other small samples of the same size were soaked in 2 U/mL elastase solution for 0,1,3,6,9,and 12 hours.To determine suitable soaking conditions for elastin-targeted enzymes and verify the digestive effect,histological staining was used to compare the effects of enzyme treatment on tissue structure and composition.The ligament samples were randomly divided into elastase-treated group and PBS group,and immersed in 2 U/mL elastase solution and PBS buffer for 6 hours,respectively.A mechanical tensile test was performed before and after immersion. RESULTS AND CONCLUSION:(1)The biochemical results showed that being treated in 2 U/mL elastase solution for 6 hours could reduce the elastin content by 31.1%,and had no significant effect on other mechanical-related components in the tissue.(2)The histological results showed that elastase was able to penetrate the tissue,and the loose degree of tissue increased after treatment.(3)In the mechanical results before and after treatment,the mechanical properties of the PBS group decreased significantly,only the low-tension elastic modulus increased significantly and the initial length increased significantly in the elastase-treated group.(4)The intergroup comparison results showed that there was no significant difference between the two groups in pre-treatment,but the low-tension elastic modulus,initial slopes,saturated slopes,and initial length of the elastase-treated group after treatment were significantly higher than those in the PBS group.(5)These results suggest that elastin degradation significantly affects the biomechanical properties of the anterior cruciate ligament and further complements our understanding of the structure-function relationship of the anterior cruciate ligament.关键词
前交叉韧带/生物力学/弹性蛋白/EVG染色/准静态拉伸/黏弹性Key words
anterior cruciate ligament/biomechanics/elastin/EVG staining/quasi-static tensile/viscoelasticity分类
临床医学引用本文复制引用
张闻天,李子涛,黄文华,邓羽平,刘晓云,李彩娟,王勉,梁泽宇,熊亮,黄刚,陈广新..弹性蛋白对前交叉韧带力学响应影响的弹性蛋白酶定量分析[J].中国组织工程研究,2024,28(22):3451-3456,6.基金项目
国家重点研发计划(2022YFB4600600),项目负责人:黄文华 （2022YFB4600600）
国家自然科学基金(31972915,32271181),项目负责人:黄文华 （31972915,32271181）
国家自然科学基金(82171966),项目负责人:李彩娟 （82171966）
广东省基础与应用基础研究基金(2020B1515120001),项目负责人:黄文华 （2020B1515120001）
中国博士后科学基金(2022M711533),项目负责人:邓羽平 （2022M711533）
广东省优秀青年人才国际培养计划博士后项目,项目负责人:邓羽平 （）
广东省医学科学技术研究基金项目(A2023129),项目负责人:熊亮 （A2023129）
黑龙江省自然科学基金项目(SS2023H004),项目负责人:李子涛 （SS2023H004）
牡丹江医学院科学基金火炬计划(2022-MYHJ-007),项目负责人:李彩娟 National Key Research&Development Plan,No.2022YFB4600600(to HWH) （2022-MYHJ-007）
National Natural Science Foundation of China,No.31972915,32271181(to HWH) （to HWH）
National Natural Science Foundation of China,No.82171966(to LCJ) （to LCJ）
Guangdong Basic and Applied Basic Research Fund,No.2020B1515120001(to HWH) （to HWH）
China Postdoctoral Science Foundation,No.2022M711533(to DYP) （to DYP）
Postdoctoral Project of the Guangdong Provincial Excellent Youth Talent International Training Program(to DYP) （to DYP）
Guangdong Medical Science and Technology Research Fund Project,No.A2023129(to XL) （to XL）
Heilongjiang Natural Science Foundation Project,No.SS2023H004(to LZT) （to LZT）
Science Foundation Torch Program of Mudanjiang Medical University,No.2022-MYHJ-007(to LCJ) （to LCJ）
