中国组织工程研究2024，Vol.28Issue(22)：3472-3477,6.DOI:10.12307/2024.563
聚丙烯酰胺改良甲基丙烯酰化明胶接枝钛合金支架的促成骨性能
Osteogenic properties of polyacrylamide-modified gelatin methacryloyl grafted titanium alloy scaffold
摘要
Abstract
BACKGROUND:Titanium and its alloys are widely used in orthopedic implants due to their excellent biocompatibility,corrosion resistance,and mechanical properties.However,it has biological inertia itself,cannot provide a good growth environment for osteoblasts,and it is difficult to form good osseointegration. OBJECTIVE:To construct a composite hydrogel material of gelatin methacryloyl and polyacrylamide on the surface of titanium alloy scaffold,and analyze its osteogenic ability in vitro. METHODS:Gelatin methacryloyl was mixed with acrylamide.Crosslinking agent and catalyst were added to synthesize gelatin methacryloyl and acrylamide(Gelma-PAAM)composite hydrogel.The titanium alloy scaffold modified by affinity silane was mixed with the Gelma hydrogel and Gelma-PAAM composite hydrogel to complete the loading(recorded as Ti-Gelma and Ti-Gelma-PAAM,respectively).The swelling ratio and degradation rate of the two hydrogels on the surface of the scaffold were compared.The bonding state between hydrogels and titanium alloy was observed by scanning electron microscope.Rat bone marrow mesenchymal stem cells were inoculated into Ti,Ti-Gelma and Ti-Gelma-PAAM scaffolds,separately.Cell proliferation,adhesion,and osteogenic differentiation were detected. RESULTS AND CONCLUSION:(1)Compared with Gelma hydrogel,Gelma-PAAM hydrogel had higher swelling rate and lower degradation rate.(2)Scanning electron microscope showed that the surface of the two kinds of hydrogels was honeycomb structure.After being combined with porous titanium alloy scaffold,the film was wrapped on the surface of scaffold and filled with pores.Among them,the Gelma-PAAM composite hydrogel coated the scaffold more fully.(3)CCK-8 assay and live/dead fluorescence staining showed that bone marrow mesenchymal stem cells proliferated well after coculture with Ti-Gelma and Ti-Gelma-PAAM scaffolds and maintained high activity.After osteogenic induction culture,alkaline phosphatase activity,calcium deposition,and osteogenic gene expression of cells of titanium alloy scaffold group were the lowest,and alkaline phosphatase activity,calcium deposition,and osteogenic gene expression of cells of Ti-Gelma-PAAM scaffold group were the highest.(4)Phalloidin cytoskeletal staining exhibited that the cells of pure titanium alloy scaffold group and Ti-Gelma scaffold group were sparse and insufficiently extended,while the cells of Ti-Gelatin-PAAM group had the most adequate stretching and the densest filamentous actin.(5)The results show that Gelma-PAAM hydrogel has good biocompatibility and osteogenic ability,and is more suitable for osteogenic modification on the surface of titanium alloy than Gelma hydrogel.关键词
水凝胶/钛合金表面改性/甲基丙烯酰化明胶/聚丙烯酰胺/成骨/骨缺损Key words
hydrogel/titanium alloy surface modification/gelatin methacryloyl/polyacrylamide/osteogenesis/bone defect分类
临床医学引用本文复制引用
褚福超,王振鑫,张大振,袁峰..聚丙烯酰胺改良甲基丙烯酰化明胶接枝钛合金支架的促成骨性能[J].中国组织工程研究,2024,28(22):3472-3477,6.基金项目
江苏省卫生健康委科研重点项目(ZD2022064),项目负责人:袁峰 （ZD2022064）
江苏省社会发展--临床前沿技术项目(BE2022708),项目负责人:袁峰 Key Scientific Research Project of Jiangsu Provincial Health Commission,No.ZD2022064(to YF) （BE2022708）
Jiangsu Provincial Social Development-Clinical Frontier Technology Project,No.BE2022708(to YF) （to YF）
