中国组织工程研究2024，Vol.28Issue(22)：3555-3560,6.DOI:10.12307/2024.537
甲基丙烯酸酐改性明胶/经处理牙本质基质生物活性支架的制备及性能
Preparation and performance of gelatin modified by methacrylic anhydride/treated dentin matrix bioactive scaffolds
摘要
Abstract
BACKGROUND:Pulp regeneration has been a hot and difficult research topic in recent years,and the construction of composite bio-scaffolding materials provides new ideas and methods for pulp regeneration. OBJECTIVE:To observe the effect of freeze-dried gelatin modified by methacrylic anhydride/treated dentin matrix bioactive scaffolds on proliferation,migration,and osteogenic differentiation of human dental pulp stem cells. METHODS:The mass ratios of gelatin modified by methacrylic anhydride and treated dentin matrix at 2:1,1:1 and 1:2 were obtained by dispersing different masses of treated dentin matrix into gelatin modified by methacrylic anhydride solution.The gelatin modified by methacrylic anhydride/treated dentin matrix bioactive scaffolds were prepared by vacuum freeze-drying.The microstructure,water absorption,and mechanical properties of the scaffolds were measured.Human dental pulp stem cells were cultured with different mass ratios of scaffold extract and DMEM(control group)to detect cell proliferation and migration.Human dental pulp stem cells were cultured with different mass ratios of scaffold extract + osteogenic induction solution and DMEM + osteogenic induction solution(control group),and their osteogenic ability was analyzed by alkaline phosphatase staining. RESULTS AND CONCLUSION:(1)Under scanning electron microscopy,the scaffolds of the three groups all had porous structures.The porosity of the scaffolds increased with the increase of treated dentin matrix quality,and there was significant difference between the two groups(P＜0.05).The water absorption of scaffolds increased with the increase of treated dentin matrix mass,and there was significant difference between groups(P＜0.05).The compressive strength and shear strength of the scaffold increased with the increase of the mass of treated dentin matrix.(2)CCK-8 assay showed that after 3,5,and 7 days of culture,the cell proliferation absorbance values in the 2:1,1:1,and 1:2 scaffold groups were higher than those in the control group(P＜0.05).The cell proliferation absorbance values increased with the increase of treated dentin matrix mass in the scaffold(P＜0.05).The cell scratch test showed that the cell migration rate in the 2:1,1:1,and 1:2 scaffold groups was higher than that in the control group(P＜0.05),and the cell migration rate increased with the increase of treated dentin matrix mass in the scaffold(P＜0.05).(3)Alkaline phosphatase staining showed that the osteogenic differentiation ability of cells in the 2:1,1:1,and 1:2 scaffold groups was stronger than that in the control group,and the osteogenic ability of cells was enhanced with the increase of treated dentin matrix mass in the scaffold.(4)The results showed that the scaffold with a mass ratio of 1:2 between gelatin modified by methacrylic anhydride and treated dentin matrix was the most suitable for the proliferation and differentiation of dental pulp stem cells.关键词
牙髓-牙本质再生/组织工程/复合生物材料/经处理牙本质基质/水凝胶/甲基丙烯酸酐明胶/人牙髓干细胞/细胞增殖分化Key words
pulp-dentin regeneration/tissue engineering/composite bio-materials/treated dentin matrix/hydrogel/gelatin modified by methacrylic anhydride/human dental pulp stem cell/cell proliferation and differentiation分类
临床医学引用本文复制引用
程梦可,杨杜娟,刘佳..甲基丙烯酸酐改性明胶/经处理牙本质基质生物活性支架的制备及性能[J].中国组织工程研究,2024,28(22):3555-3560,6.基金项目
新疆维吾尔自治区研究生实践创新项目(XJ2023G176),项目负责人:程梦可 Graduate Practice Innovation Project of Xinjiang Uygur Autonomous Region,No.XJ2023G176(to CMK) （XJ2023G176）
