中国组织工程研究2024，Vol.28Issue(27)：4367-4372,6.DOI:10.12307/2024.551
白细胞介素1B基因连锁不平衡与原发性冻结肩的易感性
Association between interleukin-1B gene linkage disequilibrium and susceptibility to primary frozen shoulder
摘要
Abstract
BACKGROUND:A large number of domestic and international documents have confirmed that elevated interleukin-1β is associated with primary frozen shoulder.Interleukin-1B gene polymorphisms can affect the transcription and protein expression of interleukin 1β-related genes,resulting in altered levels of cytokines in vivo,and thus altering the incidence of primary frozen shoulder.Through the study of interleukin-1B gene polymorphism and susceptibility to primary frozen shoulder,this study aimed to explore new breakthroughs in the pathogenesis of primary frozen shoulder from the perspective of molecular biology,and to search for susceptibility genes of primary frozen shoulder. OBJECTIVE:To explore the association between linkage disequilibrium of three gene loci in interleukin-1B gene and susceptibility to primary frozen shoulder. METHODS:A case-control study was conducted.There were two groups in this study.One group consisted of 184 patients with primary frozen shoulder,while the other group included 260 healthy controls.The genotypes of interleukin-1B gene loci-511C/T(rs16944),+3954C/T(rs1143634),and-31C/T(rs1143627)were detected by polymerase chain reaction and restriction fragment length polymorphism.The correlation between the probability of linkage disequilibrium and haplotypes and the risk of primary frozen shoulder disease was compared and analyzed. RESULTS AND CONCLUSION:Unconditional Logistic regression analysis showed that the proportion of CT genotypes at rs1143634 and rs1143627 sites increased significantly in the primary frozen shoulder.Linkage disequilibrium analysis showed that rs16944,rs1143634 and rs1143627 tended to be balanced in the control group(D'value<0.1),while there was a certain degree of linkage disequilibrium at rs1143627 and rs1143634 sites in the primary frozen shoulder group(D'value=0.595).Haplotype TTT increased the risk of primary frozen shoulder by 6.66 times compared with CCT type(TTT,OR=6.66,95%CI=1.59-27.88,P=0.009 7).To conclude,there is a certain degree of linkage disequilibrium between interleukin-1B gene loci rs1143627and rs1143634 in patients with primary frozen shoulder;haplotype TTT formed by these three gene loci may increase the risk of developing primary frozen shoulder.关键词
原发性冻结肩/白细胞介素1B/连锁不平衡/基因多态性/基因位点/基因型Key words
primary frozen shoulder/interleukin-1B/linkage disequilibrium/gene polymorphism/genetic locus/genotype分类
医药卫生引用本文复制引用
申成凯,刘坤,刘伟良,吕成昱,赵海军..白细胞介素1B基因连锁不平衡与原发性冻结肩的易感性[J].中国组织工程研究,2024,28(27):4367-4372,6.基金项目
青岛市医药卫生科研指导项目(2021-WJZD124),项目负责人:申成凯 Qingdao Medical and Health Research Guidance Program,No.2021-WJZD124(to SCK) （2021-WJZD124）
