新发传染病电子杂志2023，Vol.8Issue(6)：9-13,5.DOI:10.19871/j.cnki.xfcrbzz.2023.06.002
东莞市HIV-1流行株pol基因序列特征分析
Sequence analysis of pol gene of HIV-1 strain in Dongguan
摘要
Abstract
Objective To investigate the epidemiological characteristics of human immunodeficiency virus type 1(HIV-1)and the subtypes distribution among HIV-1-infected individuals in Dongguan.Method From January 2019 to January 2021,284 HIV-1 infected patients who had received antiviral treatment in Dongguan were selected,and was collected their epidemiological information.The viral RNA of HIV-1 was extracted from plasma.The pol gene was amplified by nested polymerase chain reaction.and gene sequence analysis was performed using biological software such as LaserGene,HIV BLAST,and MEGA 6.0.Result A total of 284 samples were collected in this study and finally successfully obtained 269 HIV-1 pol gene sequences.Among the 269 samples,86.2％(232/269)were male,61.3％(165/269)were younger than 40 years old,52.4％(141/269)were unmarried,and 49.4％(133/269)were heterosexual and 47.2％(127/269)were homosexual.Of the 269 pol gene sequences,eight genetic subtypes were identified:131(48.7％)CRF07_BC,59(21.9％)CRF01_AE,38(14.1％)CRF55_01B,19(7.1％)subtype B,12(4.5％)CRF08_BC,and 10(3.7％)other subtypes.Among 133 patients with heterosexual transmission,the predominant subtypes were 59(44.4％)CRF07_BC,32(24.1％)CRF01_AE,and 15(11.3％)CRF55_01B,and among 127 patients with homosexual transmission,the predominant subtypes were 65(51.2％)CRF07_BC,26(20.5％)CRF01_AE,and 23(18.1％)CRF55_01B.Differences in gender and marital status composition among individuals infected with different subtypes were statistically significant(P<0.05),while no statistically significant differences were observed in age and mode of infection composition.Conclusion The distribution of HIV-1 subtypes in Dongguan is complex and diverse,with CRF07_BC,CRF01_AE,and CRF55_01B being predominant.Furthermore,significant differences exist among HIV-infected individuals of different genders and marital statuses.关键词
人类免疫缺陷病毒Ⅰ型/序列分析/亚型分布Key words
Human immunodeficiency virus type Ⅰ/Sequence analysis/Subtype distribution分类
医药卫生引用本文复制引用
何松美,梁金虎,钟庆杨,曹焕焕,尹菊侦,王福祥..东莞市HIV-1流行株pol基因序列特征分析[J].新发传染病电子杂志,2023,8(6):9-13,5.基金项目
1.国家自然科学基金面上项目(81971915,82272318) （81971915,82272318）
2.深圳市科技研发基金(JCYJ20200109144203815) （JCYJ20200109144203815）
3.广东省高水平临床重点专科(深圳市配套建设经费)资助(SZGSPOI1) （深圳市配套建设经费）
4.基于干细胞技术的重大传染病临床救治方案研究(2022YFC2304403,2022YFC2304404) （2022YFC2304403,2022YFC2304404）
