miR-210-5p对小鼠脂肪干细胞成骨分化的影响及作用机制分析OACSTPCD
Effect and mechanism analysis of miR-210-5p on osteogenic differentiation of mouse adipose stem cells
[目的]观察miR-210-5p在小鼠脂肪干细胞增殖和成骨分化中的作用并探讨其作用机制.[方法]将小鼠脂肪干细胞分空白对照组、miR-210-5p模拟物阴性对照组、miR-210-5p模拟物组、miR-210-5p抑制物阴性对照组和miR-210-5p抑制物组,采用CCK-8细胞计数法检测细胞增殖能力,茜素红染色观察钙盐矿化结节形成能力,用实时荧光定量PCR检测miR-210-5p水平及成骨分化标志物BMP2、Smad1、Smad5和Runx2 …查看全部>>
[Objective]In postmenopausal osteoporosis(PMOP),adipose-derived mesenchymal stem cells(ADSCs)are unbalanced in osteogenic adipogenic differentiation,resulting in a significantly weaken osteogenic differentiation capacity.Therefore,exploring mechanisms to promote osteogenic differentiation in PMOP treatment is of great significance,and microRNA is widely involved in the regulation of bone metabolism such as osteogenesis and osteoclastic differentiation.Some s…查看全部>>
郑世雄;杨巍;刘合亮
福州市第二医院骨科,福建福州 350007福州市第二医院骨科,福建福州 350007福州市第二医院骨科,福建福州 350007
基础医学
miR-210-5pBMP2/Smad信号通路小鼠脂肪干细胞成骨分化
miR-210-5pBMP2/Smad signaling pathwaymouse adipose stem cellosteogenic differentiation
《厦门大学学报（自然科学版）》 2024 (1)
127-133,7
福建省自然科学基金(2020J011196)福建省卫生健康中青年骨干人才培养项目(2020GGB046)福建省创伤骨科急救与康复临床医学研究中心项目(2020Y2014)
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