结直肠癌组织miR-330-5p、PTBP1的表达与病理参数和预后的关系OACSTPCD

Objective To analyze the expression of microribonucleic acid-330-5p(miR-330-5p)and polypyrimidine region binding protein 1(PTBP1)in colorectal cancer tissue and their relationship with pathological parameters and progno-sis.Methods One hundred and one patients with colorectal cancer admitted to the Anorectal Department of Nantong Tra-ditional Chinese Medicine Hospital from January 2018 to May 2020 were selected.Part of the cancer tissue and its adjacent tissues were collected during surgery,and real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of miR-330-5p and PTBP1 mRNA.Predict the binding sites of miR-330-5p and PTBP1 through the Targetscan database,and analyze the differences of miR-330-5p and PTBP1 mRNA in different clinical and pathological parameters of colorectal cancer tissue;Draw its survival curve using the K-M method;Multivariate Cox regression analysis of prognostic factors in colorectal cancer patients.Results Compared with adjacent tissues,the expression of miR-330-5p in colorectal cancer tissue decreased,while the expression of PTBP1 mRNA increased(t/P=24.000/<0.001,19.233/<0.001).MiR-330-5p has a binding site with PTBP1,and their expression in colorectal cancer tissue is negatively correlated(r/P=-0.679/<0.001).Compared with low differentiation,TNM stage Ⅲ,and lymph node metastasis,colorectalcancer tissues with medi-um to high differentiation,TNM stage Ⅰ-Ⅱ,and no lymph node metastasis showed an increase in miR-330-5p and a decrease in PTBP1 mRNA expression(t/P=2.490/0.014,2.479/0.015,2.837/0.006,2.953/0.004,3.319/0.001,3.307/0.001).The overall 3-year survival rate of 101 patients with colorectal cancer was 83.17％(84/101).The 3-year overall survival rates of the miR-330-5p high expression group and the PTBP1 mRNA low expression group were higher than those of the miR-330-5p low expres-sion group and the PTBP1 mRNA high expression group,respectively(χ2/P=6.466/0.011,11.697/0.001).The independent risk factors for mortality in colorectal cancer patients are low differentiation,TNM stage Ⅲ,lymph node metastasis,and PT-BP1 mRNA≥1.50.The independent protective factors are miR-330-5p≥0.57[OR(95％CI)=3.642(1.278-10.381),3.817(1.375-10.598),4.013(1.418-11.351),2.684(1.025-7.029),0.338(0.129-0.890)].Conclusion Low expression of miR-330-5p and high expression of PTBP1 mRNA in colorectal cancer tissue are associated with differentiation degree,TNM staging,lymph node metastasis,and prognosis.