云南化工2024，Vol.51Issue(1)：38-41,4.DOI:10.3969/j.issn.1004-275X.2024.01.09
露水草中β-蜕皮甾酮提取工艺优化及其体外抗氧化能力研究
Optimization of Extraction Process of β-ecdysterone from Cyanotis Arachnoidea and Study on its Antioxidant Capacity in Vitro
摘要
Abstract
To investigate the optimal extraction technology of β-ecdysterone from Cyanotis arachnoidea,the effects of extraction method and ex-traction temperature on the content of β-ecdysterone were investigated,the content was detected by HPLC,and the antioxidant capacity of β-ecdysterone obtained by the optimal extraction technology was evaluated by DPPH and ABTS methods.The content of β-ecdysterone in extracts with water as solvent at 60℃,80℃ and 100℃ were investigated by warm soaking extraction and heated reflux extraction,and the best extrac-tion technology was selected to carry out methodological investigation.The antioxidant activity of DPPH and ABTS was determined by free radi-cal scavenging experiments in vitro.The extraction technology study showed that the content of β-ecdysterone was the highest when water was used as the solvent and reflux was heated at 100℃.The methodological results showed that under the liquid phase condition,the samples could be quickly detected with good peak shape and high separation degree.The free radical scavenging experiments showed that the antioxidant activ-ity was concentration-dependent in vitro.关键词
露水草/β-蜕皮甾酮/高效液相色谱/方法学验证/抗氧化Key words
cyanotis arachnoidea/β-ecdysterone/high performance liquid chromatography/methodology validation/antioxidant分类
医药卫生引用本文复制引用
张彦昕,柴贝贝,石玉节,李海利..露水草中β-蜕皮甾酮提取工艺优化及其体外抗氧化能力研究[J].云南化工,2024,51(1):38-41,4.基金项目
河南省科技攻关项目(182102410038) （182102410038）
郑州市高端外国专家技术合作项目(YZ22JSHZ021). （YZ22JSHZ021）
