云南化工2024，Vol.51Issue(1)：79-81,3.DOI:10.3969/j.issn.1004-275X.2024.01.19
气相色谱法测定吡唑硼酸频哪酯及方法学验证
GC Determination of Pyrazole Boronic Acid Pinacol Ester and Validation of Analytical Method
王武宝 1高思国 1刘水莲 1范荣荣 1任耀辉 1马雪 1张雨1
作者信息
- 1. 济南尚博医药股份有限公司,山东 济南 250204
- 折叠
摘要
Abstract
Objective:The GC method was established for the detection of pyrazole boronic acid pinacol ester and validation of analytical meth-od.Method:The 5％phenyl-methyl polysiloxane(30 m×φ0.25 mm,0.25 μm)column was used;The detector was FID and the carrier gas was nitrogen with a flow rate of 1.0 mL/min;The injection temperature was 215℃;The detection temperature was 295℃;The split ratio was 3:1;The injection volume was 0.1 μL;The initial column temperature was 50℃and maintained for 2 min,the column temperature was raised to 150℃ at the rate of 10℃/min and then maintained for 0 min,the column temperature was raised to 260℃ at the rate of 30℃/min and then maintained for 5 min;The H2 flow rate was 30 mL/min;The air flow rate was 300mL/min;The N2 flow rate was 25 mL/min.Result:The results of specificity,repeatability,linearity,intermediate precision and solution stability all meet the requirements.Conclusion:This method is fast and accurate that can be used for the determination of related substance of pyrazole boronic acid pinacol ester.关键词
气相色谱法/吡唑硼酸频哪酯/方法学验证Key words
GC method/pyrazole boronic acid pinacol ester/validation of analytical method分类
医药卫生引用本文复制引用
王武宝,高思国,刘水莲,范荣荣,任耀辉,马雪,张雨..气相色谱法测定吡唑硼酸频哪酯及方法学验证[J].云南化工,2024,51(1):79-81,3.
