亚热带植物科学2023，Vol.52Issue(5)：369-380,12.DOI:10.3969/j.issn.1009-7791.2023.05.001
外源独脚金内酯对烟草腋芽伸长及独脚金内酯代谢途径相关基因表达的影响
Effects of Exogenous Strigolactone on Axillary Bud Elongation and Expression of Genes Related to the Strigolactone Metabolic Pathway in Tobacco
摘要
Abstract
The effects of strigolactone(SL)on the elongation of tobacco axillary buds and the expression of genes related to its metabolic pathway were carried out,to explore the relationship between SL and the growth of tobacco axillary buds and the expression changes of SL-related genes during the elongation of tobacco axillary buds,which could establish a theoretical basis for the molecular regulation mechanism of SL in regulating the growth of tobacco axillary buds.This study investigated the developmental changes of tobacco axillary meristematic tissues after decapitation,and observed the various genes associated with SL metabolic pathway of tobacco axillary buds by treatment with GR24,an artificial synthetic analogue of SLs,using paraffin sectioning and transcriptome technique.The results showed that the axillary meristem of tobacco was composed of three cell regions:central blast region(CM),peripheral meristem(PM)and costal meristem(RM),among which the CM was located at the top of the axillary meristem,which could form PM and RM through the division of basal and lateral cells,the PM was located on both sides of the axillary meristem,and the RM was located below the CM region and the inner side of the PM region.The cell volume and arrangement of the three cell regions were different,indicating that there were differences in cell division in each part of the meristem.After axillary meristem development to form axillary buds,it was found that GR24 could inhibit the elongation of axillary buds at 1-3 nodes by exogenous application of different plant growth regulators,while BR could promote the elongation of axillary buds.Transcriptome analysis revealed that SL could affect the expression of genes related to its own metabolic pathway,SL induced the expression of D27,D14,DAD2 and SMAX1-LIKE4 compared to CK and TIS-108[a triazole-type strigolactone(SL)-biosynthesis inhibitor]treatment,whereas,D27,D14 and DAD2 were down-regulated by TIS-108 treatment,the expression of SMAX1 was inhibited by SL,while TIS-108 treatment induced the expression of SMAX1.The axillary buds of tobacco are developed from three hyphal regions of the axillary meristem,while different plant growth regulators have different effects on the axillary buds of tobacco.BR can promote the elongation of the axillary buds of tobacco,while SL can inhibit the elongation of the axillary buds,and the genes related to the metabolic pathway may play an important regulatory role in the elongation of the axillary buds of tobacco.关键词
独脚金内酯/烟草/腋芽/分生组织/基因表达Key words
strigolactone/tobacco/axillary buds/meristem/gene expression分类
农业科技引用本文复制引用
田慧源,唐博希,王原秀,刘帆,郭凯阳,刘国琴..外源独脚金内酯对烟草腋芽伸长及独脚金内酯代谢途径相关基因表达的影响[J].亚热带植物科学,2023,52(5):369-380,12.基金项目
国家自然科学基金(32060420) （32060420）
