亚热带植物科学2023，Vol.52Issue(5)：381-390,10.DOI:10.3969/j.issn.1009-7791.2023.05.002
阴香叶乙醇提取物的化学成分鉴定及其抗菌抗炎活性
Chemical Constituents and Antibacterial and Anti-inflammatory Activities of Ethanol Extracts from Cinnamomum burmanni Leaves
摘要
Abstract
Modern separation techniques such as D101 macroporous resin,MCI macroporous resin,positive and negative silica gel,gel column chromatography and semi-preparative high performance liquid chromatography were used to separate,purify and identify the chemical components in 95%ethanol extracts of Cinnamomum burmanni leaves from Meizhou,Guangdong Province.At the same time,the antibacterial activities of the compounds were determined by microplate method,and their anti-inflammatory activities were determined by LPS-induced BV2 cells.The results showed that 25 compounds were isolated and identified from the 95%ethanol extracts of C.burmanni leaves,including flavonoids(1-3),lignans(4-6),simple phenylpropanoids(7-16),coumarins(18),benzoquinones(20-21),sesquiterpenes(22-24)and indole alkaloids(25).Except for compounds 1,2,5 and 18,all of them were isolated from C.burmanni for the first time.The results of antibacterial experiments showed that compounds 20 and 21 showed good antibacterial activity against Staphylococcus aureus,with MIC of 32 μmol·L-1,while MBC of compound 20 was 64 μmol·L-1,and MBC of compound 21 was 32 μmol·L-1.The results of antibacterial kinetics also showed that compound 21 had better antibacterial activity than compound 20.The results of anti-inflammatory experiments showed that compounds 5,6 and 7 could inhibit the production of NO in BV2 cells induced by LPS,and showed good anti-inflammatory activity,the IC50 values were 7.85 μmol·L-1,24.97 μmol·L-1 and 7.49 μmol·L-1,respectively.关键词
阴香/化学成分/抗菌活性/抗炎活性Key words
Cinnamomum burmanni/chemical composition/antibacterial activity/anti-inflammatory activity分类
农业科技引用本文复制引用
苏馨科,姚敏,杨磊,吴鹏,金静维,熊平,黄俊明,李冬利..阴香叶乙醇提取物的化学成分鉴定及其抗菌抗炎活性[J].亚热带植物科学,2023,52(5):381-390,10.基金项目
广东省企业科技特派员专项(GDKTP20211049000) （GDKTP20211049000）
2021年广东省科技创新战略专项资金("大专项+任务清单")(2021A0105) （"大专项+任务清单"）
