亚热带植物科学2023，Vol.52Issue(5)：408-412,5.DOI:10.3969/j.issn.1009-7791.2023.05.006
铁甲秋海棠花粉离体萌发研究
In vitro Germination of Begonia masoniana Pollen
摘要
Abstract
To explore the suitable conditions for in vitro germination of Begonia masoniana pollen,a liquid culture method was used to investigate the effects of sucrose,boric acid,PEG-4000,calcium chloride concentration,and different cultivation times on pollen tube growth through single factor experiments.The results showed that the optimal medium for pollen germination was sucrose 5%,boric acid 0.01%,PEG-4000 5%,calcium chloride 0.003%.The optimal observation time was 120 min.The pollen germination rate of B.masoniana was 90.86%.The length of the pollen tube was 511.51μm.Our results could provide reference for pollen research and breeding of other begonias.关键词
铁甲秋海棠/花粉萌发/花粉管生长/离体培养Key words
Begonia masoniana/pollen germination/pollen tube growth/culture in vitro分类
农业科技引用本文复制引用
吕燕玲..铁甲秋海棠花粉离体萌发研究[J].亚热带植物科学,2023,52(5):408-412,5.基金项目
厦门市社会发展领域指导性项目(3502Z20234ZD4001) （3502Z20234ZD4001）
