空军军医大学学报2024，Vol.45Issue(1)：5-8,4.DOI:10.13276/j.issn.2097-1656.2024.01.002
异种肝肾联合移植中猪内源性逆转录病毒的检测
Detection of porcine endogenous retrovirus in combined liver and kidney xenotransplantation
摘要
Abstract
Objective To detect the expression of porcine endogenous retrovirus(PERV)genes in donor and recipient of combined liver and kidney xenotransplantation,hoping to provide a reference for the cross-species transmission of PERV.Methods The tissue samples of 6-gene-edited pig-to-rhesus macaque combined liver and kidney xenotransplantation were selected as research objects.The expressions of PERV coding gene,PERV subtypes,and porcine mitochondrial cytochrome B gene in different tissues of donor and recipient samples during xenotransplantation were detected by RT-PCR and PCR.Results There were PERV coding genes gag and pol,PERV-A and PERV-B subtypes,and porcine mitochondrial cytochrome B gene in different tissues of donor pig,but no PERV-C subtype was detected in the donor tissue samples.At the end of transplantation,no PERV gene sequences were detected in different tissues of the recipient.Conclusion No cross-species transmission of PERV occurred within 14 d of survival in gene-edited pig-to-rhesus macaque combined liver and kidney xenotransplantation.关键词
异种器官移植/猪内源性逆转录病毒/人畜共患病/跨物种传播Key words
xenotransplantation/porcine endogenous retrovirus/zoonotic diseases/cross-species transmission分类
医药卫生引用本文复制引用
张虹,张玄,陶开山,窦科峰..异种肝肾联合移植中猪内源性逆转录病毒的检测[J].空军军医大学学报,2024,45(1):5-8,4.基金项目
国家自然科学基金(82070671,82371793,82170667) （82070671,82371793,82170667）
