空军军医大学学报2024，Vol.45Issue(1)：9-14,6.DOI:10.13276/j.issn.2097-1656.2024.01.003
国际首例6-基因编辑猪-猴肝肾联合移植围术期治疗研究
Perioperative treatment of the first international 6-gene-edited pig-to-rhesus macaque combined liver and kidney transplantation
摘要
Abstract
Objective To investigate the establishment of the first international 6-gene-edited pig-to-rhesus macaque combined liver and kidney transplantation model,and to explore the influence of liver xenograft on kidney xenograft.Methods The liver and kidney of humanized 6-gene-edited pig were transplanted to rhesus recipient.Heterotopic abdominal transplantation was used for liver transplantation and orthotopic transplantation for kidney transplantation,with the contralateral kidney retained.The condition of the transplanted liver and kidney and the recipient survival were observed after blood perfusion recovery.Regular graft ultrasound was performed to examine the function of grafts.The changes of biochemical and physiological indexes were monitored.At the end of life of the recipient,the transplanted kidney and liver were taken for pathological examination.Results The operation lasted for 14 h and the physiological indexes of the recipient were basically normal after operation.After the open blood flow of the grafts,the transplanted liver was well perfused and bile outflow was immediately visible.The perfusion of the transplanted kidney was good,urine outflow could be seen immediately,and no obvious hyperacute rejection was observed.After operation,the transplanted kidney function was good,and the urine volume of the transplanted kidney decreased 5 d after operation attributing to rejection reaction.The recipient survived for 14 d after operation.Conclusion The multi-gene-edited pig-to-rhesus macaque combined liver and kidney transplantation model was successfully constructed,and no obvious hyperacute rejection was observed in the transplanted kidney after operation.Xenograft liver transplantation may alleviate the acute rejection of xenograft kidney transplantation.关键词
异种移植/基因编辑猪/肝肾联合移植/排斥反应Key words
xenotransplantation/gene-edited pigs/combined liver and kidney transplantation/rejection reaction分类
医药卫生引用本文复制引用
韩士超,杨晓剑,陶开山,秦卫军,窦科峰,马帅军,戚若晨,王国辉,张小燕,刘克普,范效铮,魏迪,张玄..国际首例6-基因编辑猪-猴肝肾联合移植围术期治疗研究[J].空军军医大学学报,2024,45(1):9-14,6.基金项目
国家自然科学基金(82102322) （82102322）
