空军军医大学学报2024，Vol.45Issue(1)：26-31,6.DOI:10.13276/j.issn.2097-1656.2024.01.006
猪-恒河猴心脏异种移植的围术期受体感染预防及治疗策略总结与分析
Summary and analysis of perioperative strategies for preventing and treating recipient infections in pig-to-rhesus heart xenotransplantation
摘要
Abstract
Objective To summarize and analyze the perioperative recipient infection and treatment strategies according to the gene-edited pig-to-rhesus heart xenotransplantation in 2022,so as to provide guidance for perioperative infection control of xenotransplantation.Methods The experiment utilized male gene-edited(GTKO/β4GalNT2 KO/CMAH KO/hCD55/hCD46/hTBM)pig as donor and male rhesus as recipient.Pig heart was ectopically transplanted into the abdominal cavities of the recipient.Perioperative intensive care was given to monitor infection indicators including signs,blood routine,serum inflammatory factors,chest radiographs,and bacterial cultures(blood,drainage,secretions,and excretions).The use of anti-infective drugs was adjusted based on changes in these indicators.Results The recipient rhesus survived and the transplanted heart maintained systolic function for 20 d,with minimal fluctuations in body temperature and blood routine,no hyperthermia(＞38.5 ℃)or elevated white blood cell counts.On the 9 th day after surgery,chest X-rays revealed patchy shadows resembling infection,which was improved after adjusting antibiotic usage and strengthening lung care.Postoperative inflammatory indicators,such as C-reactive protein and procalcitonin,did not show significant increases.However,on the 13th day after surgery,bacterial cultures from oral secretions,ascites,and gastric juice were positive for Staphylococcus haemolyticus.Conclusion The high-intensity and high-dose use of immunosuppressive drugs leads to an increased incidence of recipient infections following xenotransplantation.Nonetheless,the timely examination and adjustment of treatment strategies can effectively control the progression of these infections.关键词
异种心脏移植/基因编辑猪/恒河猴/术后感染Key words
heart xenotransplantation/gene-edited pig/rhesus/postoperative infection分类
医药卫生引用本文复制引用
周晔农,段维勋,刘金成,金振晓,张冰,陈涛,杨秀玲,金艳,吕向妮,郭红,易蔚,赵荣..猪-恒河猴心脏异种移植的围术期受体感染预防及治疗策略总结与分析[J].空军军医大学学报,2024,45(1):26-31,6.基金项目
国家自然科学基金面上项目(82370273) （82370273）
空军军医大学西京医院临床新技术项目(XJSZ03,XJSM02,XJSM14,XJSM15) （XJSZ03,XJSM02,XJSM14,XJSM15）
空军军医大学西京医院学科助推计划项目(XJZT23XG28) （XJZT23XG28）
