七氟醚预处理和后处理对局灶性脑缺血再灌注损伤大鼠神经元坏死性凋亡的影响OA

Objective To establish a rat model of focal cerebral ischemia-reperfusion injury by modified Zea-longa suture method,observe the effect of sevoflurane treatment on neurons of focal cerebral ischemia-reperfusion injury rats,and explore its mechanism.Methods Adult male Sprague-Dawley(SD)rats were randomly divided into 4 groups:Sham group,ischemia reperfusion(IR)group,sevoflurane pre-treatment(Spre)group and sevoflurane post-treatment(Spo)group,with 10 rats in each group.After the modeling,the Longa score method was used to evaluate neuroethology.Enzyme-linked immunosorbent assay(ELISA)was used to detect the activities of superoxide dismutase(SOD)and the levels of malondialdehyde(MDA)in rat brain tissue.Western blotting was used to detect the expression of NADPH oxidase 4(NOX4)and Caspase3 proteins.The cell apoptosis was detected by flow cytometry.Results Compared with Sham group,the neurobehavioral score,MDA levels,expressions of NOX4 and Caspase3 and neuronal apoptosis number in IR group were significantly increased,while the SOD activity was decreased,with statistically significant differences(P＜0.05).Compared with IR group,the neurobehavioral scores,MDA levels,expressions of NOX4 and Caspase3 and neuronal apoptosis number in Spre group and Spo group were significantly reduced,while the SOD activity was decreased,with statistically significant differences(P＜0.05).Conclusion In the modle of focal cerebral ischemia-reperfusion injury in rats,both pre-treatment and post-treatment with sevoflurane have certain protective effects on brain tissue of rats with ischemia-reperfusion injury.The protective effects may be achieved by inhibiting neuronal necrotic apoptosis caused by oxidative stress injury.