临床研究2024，Vol.32Issue(2)：1-4,4.DOI:10.12385/j.issn.2096-1278(2024)02-0001-04
七氟醚预处理和后处理对局灶性脑缺血再灌注损伤大鼠神经元坏死性凋亡的影响
Effects of Sevoflurane Pretreatment and Post-treatment on Necrotizing Apoptosis of Neurons in Rats with Focal Cerebral Ischemia-reperfusion Injury
摘要
Abstract
Objective To establish a rat model of focal cerebral ischemia-reperfusion injury by modified Zea-longa suture method,observe the effect of sevoflurane treatment on neurons of focal cerebral ischemia-reperfusion injury rats,and explore its mechanism.Methods Adult male Sprague-Dawley(SD)rats were randomly divided into 4 groups:Sham group,ischemia reperfusion(IR)group,sevoflurane pre-treatment(Spre)group and sevoflurane post-treatment(Spo)group,with 10 rats in each group.After the modeling,the Longa score method was used to evaluate neuroethology.Enzyme-linked immunosorbent assay(ELISA)was used to detect the activities of superoxide dismutase(SOD)and the levels of malondialdehyde(MDA)in rat brain tissue.Western blotting was used to detect the expression of NADPH oxidase 4(NOX4)and Caspase3 proteins.The cell apoptosis was detected by flow cytometry.Results Compared with Sham group,the neurobehavioral score,MDA levels,expressions of NOX4 and Caspase3 and neuronal apoptosis number in IR group were significantly increased,while the SOD activity was decreased,with statistically significant differences(P＜0.05).Compared with IR group,the neurobehavioral scores,MDA levels,expressions of NOX4 and Caspase3 and neuronal apoptosis number in Spre group and Spo group were significantly reduced,while the SOD activity was decreased,with statistically significant differences(P＜0.05).Conclusion In the modle of focal cerebral ischemia-reperfusion injury in rats,both pre-treatment and post-treatment with sevoflurane have certain protective effects on brain tissue of rats with ischemia-reperfusion injury.The protective effects may be achieved by inhibiting neuronal necrotic apoptosis caused by oxidative stress injury.关键词
七氟醚,预处理,后处理,脑缺血再灌注损伤,神经元/凋亡Key words
sevoflurane/pretreatment/post-treatment/cerebral ischemia-reperfusion injury/neurons/apoptosis分类
医药卫生引用本文复制引用
王海丽,杨爱玲,刘兆东,郭达茹,潘华..七氟醚预处理和后处理对局灶性脑缺血再灌注损伤大鼠神经元坏死性凋亡的影响[J].临床研究,2024,32(2):1-4,4.基金项目
七氟醚预处理和后处理对局灶性脑缺血—再灌注损伤大鼠神经元坏死性凋亡的影响(LHGJ20210969). （LHGJ20210969）
