大豆卵磷脂对绵羊精液低温保存效果的影响OA

In order to study the effect of soybean lecithin on the cryopreservation of Dupo sheep semen,the trial added 0(control),1.875,3.750,7.500,15.000 g/L of soy lecithin to Dupo sheep semen dilution and stored at low temperature.Semen motility was observed every 12 hours,the effective survival time and total survival time of semen were counted,and the sperm malformation rate and acrosome integrity rate were measured at the 48th hour.The results showed that the effective survival time and overall survival time of the 1.875 g/L group were significantly higher than those in the control group and the rest of the experimental groups(P<0.05).The acrosome integrity rate of the 1.875 g/L group was significantly higher than that of the 15.000 g/L group(P<0.05),and the malformation rate was significantly lower than that of the 15.000 g/L group(P<0.05).In summary,the adding 1.875 g/L of soybean lecithin to the dilution could prolong the survival time of sperm and improve the quality of cryopreservation of sheep semen.