中国稻米2024，Vol.30Issue(1)：47-52,6.DOI:10.3969/j.issn.1006-8082.2024.01.008
外源氨基酸对层出镰刀菌菌丝生长和伏马毒素合成的影响
Effects of Exogenous Amino Acids on the Mycelial Growth and Fumonisins Production of Fusarium proliferatum
摘要
Abstract
Rice spikelet rot disease caused by Fusarium proliferatum not only leads to yield reduction,but also contaminates rice grains with mycotoxins.In this study,the effects of exogenous amino acids on the mycelial growth and fumonisins biosynthesis of F.proliferatum were determined through the addition of amino acids to the growth media.The results showed that the growth of F.proliferatum was affected by different exogenous additives and different addition amounts compared to the mock control.The mycelial growth of F.proliferatum was obviously inhibited in MM media supplied with L-arginine,L-glutamate,L-proline and L-citrulline,respectively,and in PDA media supplied with L-glutamate.Fumonisins production was significantly increased by different kinds of amino acids.Fumonisins content tended to reduce in PDA media and increase in MM media with the higher concentration of amino acids.There were significant changes in expression levels of the fumonisins biosynthetic(fum)genes treated with exogenous amino acids,but expression patterns of fum genes were not consistent with fumonisins production.The results demonstrated that the mycelial growth and fumonisins production of F.proliferatum were influenced by the quantity and type of amino acids,and the correlation between different amino acid composition and mycelial growth fumonisins biosynthesis was different.关键词
水稻/穗腐病/层出镰刀菌/外源氨基酸/菌丝生长/伏马毒素Key words
rice/spikelet rot disease/Fusarium proliferatum/exogenous amino acids/mycelial growth/fumonisins分类
农业科技引用本文复制引用
梁文豪,胡培松,胡时开,圣忠华,魏祥进,焦桂爱,邵高能,谢黎虹,王玲,唐绍清..外源氨基酸对层出镰刀菌菌丝生长和伏马毒素合成的影响[J].中国稻米,2024,30(1):47-52,6.基金项目
国家自然科学基金(32188102,31800133) （32188102,31800133）
浙江省重点研发项目(2021C02063,2021C02056,2022C02011) （2021C02063,2021C02056,2022C02011）
