基于间接波前整形的近红外二区荧光共聚焦成像研究
NIR-Ⅱ fluorescence confocal imaging based on indirect wavefront shaping
摘要
Abstract
Optical aberrations caused by the scattering of biological tissues limit the imaging performance of optical systems.A near-infrared Ⅱ fluorescence confocal imaging technique based on indirect wavefront shaping was investigated.First,we synthesized a highly efficient near-infrared Ⅱ range fluorescent probe,where reducing the scattering of biological tissue can realize biopsy imaging with high-contrast.Second,we investigated the adaptive optical method based on indirect wavefront measurement.The indirect wavefront shaping technology was applied to the laser scanning confocal system,enabling the measurement and com-pensation of optical aberrations caused by biological tissues,and obtaining imaging of biological tissues with a high signal-to-noise ratio.Finally,near-infrared Ⅱ fluorescence confocal imaging system based on indirect wavefront shaping was deployed and relevant experiments were conducted.The experimental results indic-ate that the system effectively compensates for the aberrations induced by air plates,scattering media and mouse skull,and increases the final signal intensity by 1.47,1.95 and 2.85 times,respectively.As a result,the final imaging quality is significantly enhanced.关键词
间接波前整形/近红外二区成像/共聚焦成像/活体实验Key words
Indirect wavefront shaping/near-infrared-Ⅱ imaging/confocal imaging/in vivo experiments分类
数理科学引用本文复制引用
谭天,史天悦,吴长锋,彭洪尚..基于间接波前整形的近红外二区荧光共聚焦成像研究[J].中国光学（中英文）,2024,17(1):150-159,10.基金项目
国家自然科学基金(No.62175266,No.62235007,No.22204070) （No.62175266,No.62235007,No.22204070）
深圳市科技计划项目(No.KQTD201708 10111314625,No.JCYJ20210324115807021,No.SGDX20211123114002003) （No.KQTD201708 10111314625,No.JCYJ20210324115807021,No.SGDX20211123114002003）
深圳湾实验室开放课题(No.SZBL2021080601002) （No.SZBL2021080601002）
广东省先进生物材料重点实验室(No.2022B1212010003)Supported by the National Natural Science Foundation of China(No.62175266,No.62235007,No.22204070) （No.2022B1212010003）
the Shenzhen Science and Technology Program(No.KQTD20170810111314625,No.JCYJ2021 0324115807021,No.SGDX20211123114002003) （No.KQTD20170810111314625,No.JCYJ2021 0324115807021,No.SGDX20211123114002003）
the Shenzhen Bay Laboratory(No.SZBL2021080601002) （No.SZBL2021080601002）
Guangdong Provincial Key Laboratory of Advanced Biomaterials(No.2022B1212010003) （No.2022B1212010003）
