局解手术学杂志2024，Vol.33Issue(2)：100-106,7.DOI:10.11659/jjssx.01E023037
高压电击伤小鼠的血液代谢组学研究
Metabonomic study of blood of mice with high-voltage electrical injury
摘要
Abstract
Objective To explore the changes of metabonomics in blood of mice after high-voltage electric shock,then screen out the significantly changed differential metabolites and metabolic pathways.Methods The head of C57BL/6J mice was subjected to high-voltage electric shock(electric shock group)or exposed to acoustic and optical stimulation of high-voltage electric(control group),then the whole blood from mice were collected to separate serum.The dual platform combined metabonomic analysis based on gas chromatography-mass spectrometer(GC-MS)and liquid chromatography-mass spectrometer(LC-MS)was performed and orthogonal partial least squares discriminant analysis(OPLS-DA)was used to screen the differential metabolites and related metabolic pathways.Results A total of 415 differential metabolites were screened out in metabonomics in blood of mice after high-voltage electric shock,including 187 up-regulated and 228 down-regulated metabolites.These differentially metabolites were significantly enriched in metabolic pathways including central carbon metabolism in cancer,glucagon signaling pathway,etc.Conclusion By establishing the model of high-voltage electrical injury on experimental mice,this study reveals the significant change of metabolite content and metabolic pathway in blood by high-voltage electrical injury.Which provides a basis for the damage of blood metabolic activity by high-voltage electrical injury,and suggests the potential harm of high-voltage electrical injury to blood metabolic activity in the whole body.关键词
电工作业/高压电击伤/血液/血生化/代谢组学Key words
electrician operation/high-voltage electrical injury/blood/blood biochemistry/metabolomics分类
医药卫生引用本文复制引用
陈思羽,王徽,罗燕,陶嘉雯,张文娟,岳洋,余争平,皮会丰..高压电击伤小鼠的血液代谢组学研究[J].局解手术学杂志,2024,33(2):100-106,7.基金项目
重庆市科卫联合医学科研项目(重大项目)(2023DBXM006) （重大项目）
