中国临床药理学杂志2024，Vol.40Issue(1)：37-41,5.DOI:10.13699/j.cnki.1001-6821.2024.01.008
微小RNA-133a-3p对乳腺癌细胞的影响及机制研究
Effect and mechanism of microRNA-133A-3p on breast cancer cells
摘要
Abstract
Objective To explore the effect and mechanism of microRNA-133a-3p(miR-133a-3p)on invasion and apoptosis of breast cancer cells through targeted regulation of cullin-associated NEDD8-dissociated 1(CAND1).Methods MCF-7 cells were divided into overexpression group(mimics miR-133 a-3p transfection),NC group(mimics control transfection),co-transfection group(mimics miR-133a-3p transfection with pcDNA-CAND1 co-transfection)and control group(only adding the same amount of transfection reagents).Flow cytometry was used to detect cell apoptosis,Transwell assay was used to detect cell invasion,and real-time fluorescence quantitative polymerase chain reaction was used to detect miR-133a-3p and CAND1 expression.Results After transfection,the expression levels of miR-133a-3p in control group,NC group,overexpression group and co-transfection group were 0.50±0.08,0.51±0.09,1.06±0.10 and 1.05±0.15,respectively;the expression of CAND1 mRNA were 0.91±0.09,0.91±0.07,0.80±0.10 and 1.21±0.10,respectively.There were statistically significant differences in the above indexes between the co-transfection group and the control group,the NC group(all P＜0.05),and there were statistically significant differences between the overexpression group and the control group,the NC group(all P＜0.05).The apoptosis rates in control group,NC group,overexpression group and co-transfection group were(7.88±1.62)％,(8.87±2.01)％,(53.41±5.46)％,(29.54±3.78)％,respectively.The number of invasive cells in control group,NC group,overexpression group and co-transfection group were 161.02±10.31,155.87±12.30,85.21±9.11 and 118.37±10.84,respectively.There were statistically significant differences in the above indexes between transfection group and overexpression group,control group and NC group(all P＜0.05),and there were statistically significant differences between overexpression group and control group and NC group(all P＜0.05).Conclusion Overexpression of miR-133a-3p in human breast cancer cells MCF-7 can inhibit CAND1 and promote apoptosis and invasion of MCF-7 cells.关键词
微小RNA-133a-3p/乳腺癌/侵袭/凋亡Key words
microRNA-133 a-3p/breast cancer/invade/apoptosis分类
医药卫生引用本文复制引用
田彩平,闵建平,荆晓,杨碎胜..微小RNA-133a-3p对乳腺癌细胞的影响及机制研究[J].中国临床药理学杂志,2024,40(1):37-41,5.基金项目
甘肃省卫生行业科研计划基金资助项目(GSWSKY-2019-35) （GSWSKY-2019-35）
甘肃省科技计划基金资助项目(22JR5RA643) （22JR5RA643）
甘肃省卫生行业科研管理基金资助项目(GSWSKY-2019-96) （GSWSKY-2019-96）
兰州市科技发展指导性计划基金资助项目(2020-ZD-52) （2020-ZD-52）
